Protein Info for PfGW456L13_2355 in Pseudomonas fluorescens GW456-L13

Annotation: sigma-54 dependent transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 PF01590: GAF" amino acids 124 to 215 (92 residues), 34.3 bits, see alignment E=9.9e-12 PF00158: Sigma54_activat" amino acids 342 to 508 (167 residues), 214.6 bits, see alignment E=2.3e-67 PF14532: Sigma54_activ_2" amino acids 360 to 513 (154 residues), 68.9 bits, see alignment E=1.7e-22 PF07728: AAA_5" amino acids 364 to 475 (112 residues), 31.1 bits, see alignment E=6.7e-11 PF02954: HTH_8" amino acids 599 to 639 (41 residues), 47.7 bits, see alignment 3.1e-16

Best Hits

KEGG orthology group: None (inferred from 81% identity to pfl:PFL_4135)

Predicted SEED Role

"sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QM27 at UniProt or InterPro

Protein Sequence (641 amino acids)

>PfGW456L13_2355 sigma-54 dependent transcriptional regulator (Pseudomonas fluorescens GW456-L13)
VDDPERLMTPIKTNSPKLHREARLAREKLHLEGEVPDGVLRAEIDASWRRSLSHGVHFNA
KHELALESSASLDVLLASNRLLIDAALPAIDYLAERQGKEGLIILANSDATILAVEGRAD
RLKGSGLQDITLGTCWSEAVRGTNALGTALVEARPTLIDCGEHYLDRLSDFSCTSVPIHC
PQGDILGVLDLTREGPLGRAHDSTALLAMAVSQIESRVFNASYPDEIVLAFHSRRQYLES
PWQGLLAVSLGGQILAVSAQACQLLHAERSALVGRRCEEFLGVDGLQLLARLHQGGAGSL
QTAKGEFFYKTLRAPQRSINVSSPPRTVAKPAKAQPDLEALAGSNTRYARALRMARQGLA
NELPVLLLGETGTGKEVIARALHMAGARCDKPFVAVNCAAIPEGLIESELFGYREGAFTG
SRRGGMVGRLQQAHGGTLFLDEIGDMPLALQARLLRVLQDRKVAPLGAGEEQDIDVALIC
ATHRDLKRLVEEKHFREDLFYRVNGISVMLPALREREDFGALVTRMLTTLGAPAVVLHDD
LDRLLNGYQWPGNIRQLEMVLRTALAMREPGDTVLTLDHLPDSMLDELTTSERPQAGSIR
ENELELIRQSLDTHQGNVSAAADALGISRATLYRKLKQLRS