Protein Info for PfGW456L13_2260 in Pseudomonas fluorescens GW456-L13

Annotation: Hybrid sensory histidine kinase in two-component regulatory system with EvgA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1209 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 535 to 555 (21 residues), see Phobius details PF00497: SBP_bac_3" amino acids 62 to 272 (211 residues), 92.9 bits, see alignment E=7.8e-30 amino acids 301 to 519 (219 residues), 109.1 bits, see alignment E=8.2e-35 TIGR00229: PAS domain S-box protein" amino acids 576 to 694 (119 residues), 26.3 bits, see alignment E=3.5e-10 PF00989: PAS" amino acids 576 to 688 (113 residues), 26.7 bits, see alignment E=1.7e-09 PF08448: PAS_4" amino acids 583 to 693 (111 residues), 43.6 bits, see alignment E=1.1e-14 PF00512: HisKA" amino acids 711 to 777 (67 residues), 61.5 bits, see alignment 2.2e-20 PF02518: HATPase_c" amino acids 826 to 941 (116 residues), 95.6 bits, see alignment E=9e-31 PF00072: Response_reg" amino acids 965 to 1078 (114 residues), 83.1 bits, see alignment E=5.7e-27 PF01627: Hpt" amino acids 1110 to 1192 (83 residues), 38.5 bits, see alignment 3.8e-13

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 71% identity to pfo:Pfl01_3975)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293PZE7 at UniProt or InterPro

Protein Sequence (1209 amino acids)

>PfGW456L13_2260 Hybrid sensory histidine kinase in two-component regulatory system with EvgA (Pseudomonas fluorescens GW456-L13)
LKVYLIQLTAGLCVSTSLFGAPGNENYTLLSRSKVGALEVRLDESQRQWIKQKNQLTLGT
SAPDYPPFDLTTSGQDYEGFTADYAGLLEKVTGLPVTVQRFDSRGAAITALENGEVDLLG
TANGFEAGNADIALSIPYAVDQPVLVTREGETRSLTDGLAGLRLSMVYHYLPLAEVKALY
PKAIITSYPSYQNAINAVAFDQADVFLGDTISTHYMINKGYLNNIRMANFGKHEAFGFSF
AVNRNNPELLGIINSMITAIPSSERENIAKRWSAGSDILLTDQKLQLTDREERWLSHHPV
VRVVVNEAFAPLTFFDSNGNFRGVTADLLELIRLRTGLRFDIRSSRSDDEMIKQIKDNKV
DVIAALLPSAERQTQLNFTRPFLENSYVLLTRKAADSSTNLAQLQDKRLAIARGNPIIDY
LRREFPRINLIETPDTFSAVELLAEGQAEGAVNSLVIANYFISSRLFEHTLQISTTIGTE
QAAFSLATARGAKELNAILDKALLSISPEELGIINSRWRGYSASSQSTWRNYQRLFYQIV
IGAGVLLLISIAWNDYMRRQIKQRKAAERALNDQFEFMRSLVNGTPHPIYVRDRQGLLQS
CNDSYLQAFCARREDVIGKSVMQGSMSNVFEAREYQADYQRVVAEGRPLIVDRELHIGGR
RLTIYHWILPYRDSSAKVQGIIGGWIDISERRQLLDDLRCAKERADNANRAKSTFLATMS
HEIRTPMNAIIGMLELTLKRLDHQHPERPAIDVAYNSANGLLELIGDILDIARIESGRLS
LTPERVDPGELVTSVVRIFEGLARQKELELMLEFSSANPTFDVLLDPLRFKQVLSNLISN
AIKFTEQGQVRVIVDLHPATQPEHVQMRVQVHDSGIGISEQDQQRLFEPFAQADHAMQSA
RGGAGLGLVISRNLCEMMGGSLQLRSEPGIGTQVEVLLQLETLPLQQSARAVETQVVVAT
TPLNVLVVDDHSANRLLTCQQLEFLGHRFTVAADGQEGLEAWKDQPFDLVIADCNMPVMN
GYELARAIRQHERQLHRPPCTVLGFTANAQPEEIQRCKQAGMDDCLFKPLSLGALSQWVQ
GIEPTHVAPAFSLHGLKLLTGGNPKLNRRLLNELVNSNRQDLPTLLSLSSSGDTQALQDI
AHKIKGAARIVQASRLIDRCEALEAACHEDSDREKVAGCCKSMERTMLELEDALLRQIEH
YDEGKLPEP