Protein Info for PfGW456L13_2204 in Pseudomonas fluorescens GW456-L13

Annotation: Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 883 transmembrane" amino acids 385 to 407 (23 residues), see Phobius details amino acids 412 to 429 (18 residues), see Phobius details amino acids 436 to 454 (19 residues), see Phobius details amino acids 466 to 484 (19 residues), see Phobius details PF02702: KdpD" amino acids 19 to 227 (209 residues), 306.5 bits, see alignment E=2.1e-95 PF00582: Usp" amino acids 248 to 360 (113 residues), 42.9 bits, see alignment E=1.8e-14 PF13493: DUF4118" amino acids 390 to 494 (105 residues), 92.1 bits, see alignment E=4.5e-30 PF00512: HisKA" amino acids 652 to 719 (68 residues), 55.4 bits, see alignment 1.3e-18 PF02518: HATPase_c" amino acids 767 to 874 (108 residues), 89.2 bits, see alignment E=6.3e-29

Best Hits

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 94% identity to pfo:Pfl01_4030)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QP81 at UniProt or InterPro

Protein Sequence (883 amino acids)

>PfGW456L13_2204 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) (Pseudomonas fluorescens GW456-L13)
MSNSGRADALLADLPRDGRGRLKVFLGAAPGVGKTYAMLQSAHTQLRQGVKVIAGVVETH
GRAETEALLGGLSQQPLVRSEYRGVLLEEMDLDGLLLAKPKLVLVDELAHTNAPGSRHEK
RWQDIQELLAAGIDVYTTVNVQHLESLNDQVRGITGVQVRETLPDWVLQEAYELLLIDLP
PRELLERLRDGKVYVPEQARAAIDAFFSQTNLTALRELAMQTAAAQVDNDLAQGYRQLGQ
AAPAVRGRLLVGVDGDAQAERLVRHASRVAQRRHLPWSLVHVDNGSVRDEQSRLRLQSAQ
QLAERLGGEVVLLRAGEVAKTLIQHAAERRASLVLVGQSRPRLRRRLFGGGLAARLLRNA
RGLEINVLDSDHQPHQPHRRSMHSLVWFDYALAVVATISASALAWAVASVLPLPNISLVF
LAAVLLVAVRSSLGPALACAVLSFLTYDFLFIPPNFSFSIQREEDVLTLLFFLLMAALTG
NLAARQRRQLQALRDTQEETTELLDLSRKLTAATDRQAVVSAATQHLNGWSDVQLCLLNR
DGQNGWKVETGGPLEFTEAERAAADWAWQHDQPAGAGTGTLPFGRWWWWPLSVEDGPLAL
LGVCARDGQTLSGQRRRLLTALTQPLAQALARAQLADDLEAARLHGETEQLRSALLASVS
HDLRTPLTSMRGSIDSLLALGEAIPLEDRRELLEGTRDEAERLDRYIQNLLDMTRLGHGA
LKLARDWVSPADIVGSALNRLRTVLAPLQVSTDVPAELPLLFVHAALIEQALINVMENAA
RFSPPRGRLQLHAGADDSELFFSVSDEGPGIPEEDRAKIFDMFYTAARGDRGGQGTGLGL
AICQGMVGAHGGRISVADGIEGRGTCITLHLPLQAQPGYESEA