Protein Info for PfGW456L13_2155 in Pseudomonas fluorescens GW456-L13

Annotation: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 659 PF05430: Methyltransf_30" amino acids 114 to 233 (120 residues), 136.7 bits, see alignment E=9.8e-44 PF01266: DAO" amino acids 264 to 629 (366 residues), 172.2 bits, see alignment E=6.1e-54 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 264 to 658 (395 residues), 457.9 bits, see alignment E=1.6e-141 PF00890: FAD_binding_2" amino acids 264 to 295 (32 residues), 21.3 bits, see alignment (E = 3.6e-08) PF13450: NAD_binding_8" amino acids 266 to 298 (33 residues), 27.1 bits, see alignment (E = 1e-09)

Best Hits

Swiss-Prot: 86% identical to MNMC_PSEPF: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: None (inferred from 86% identity to pfo:Pfl01_4098)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QNU4 at UniProt or InterPro

Protein Sequence (659 amino acids)

>PfGW456L13_2155 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase (Pseudomonas fluorescens GW456-L13)
MKPVLPHAQLDWDDQGRPYSRVFDDVYFSDKSGLDETRYVFIEQNRLAERFAALPEGGRL
VIGETGFGTGLNFLCAWQVFEQHAAAGARLHFVSVEKYPLSPADLQRALALWPELKVFAD
QLLEQYVAIHQGFQRLTLDHGRVTLTLLIGDALEQLPQLDARIDAWFLDGFAPAKNPDMW
TAELFAELARLAAPGASISTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGEFLGWPL
ETPAPALDKPWFARPAPLTGERQALVIGAGLAGCASAASLAARGWQVTLLERHDAIAREA
SGNPQGVLYLKLSAHGTALSQMIVSGFGYTRRLLEHLQRGRDWDDCGVLQLAFNAKEAER
QAQLAAAFPVDLLHLLDQPEAQAVAGIGLAHGGLFYPEGGWVHPPALCQWQAAHPLIQLL
THHDALELRKVEGRWQAWDGERLLADAPVVVLAGAAEIQRFPESAELPLKRIRGQITRLA
QTPQSQALSTVVCAEGYVAPARLGEHTLGASFDFKSDDLTPTTAEHLGNLAMLEEISHDL
VERLQADKLAPESLQGRAAFRCTSPDYLPIVGPLADTQAFTQAYAALGKDARQVPDVECP
WIDGLYINSGHGSRGLITAPLSAELLAAWLDNEPLPLPRSVAEACHPNRFALRRLIRGK