Protein Info for PfGW456L13_213 in Pseudomonas fluorescens GW456-L13

Annotation: Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 979 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF03710: GlnE" amino acids 45 to 295 (251 residues), 281.4 bits, see alignment E=6.5e-88 amino acids 574 to 827 (254 residues), 312.8 bits, see alignment E=1.7e-97 PF08335: GlnD_UR_UTase" amino acids 315 to 453 (139 residues), 106.3 bits, see alignment E=1.4e-34 amino acids 872 to 947 (76 residues), 35.5 bits, see alignment 1e-12

Best Hits

Swiss-Prot: 82% identical to GLNE_PSESM: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 93% identity to pfo:Pfl01_0464)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QIY9 at UniProt or InterPro

Protein Sequence (979 amino acids)

>PfGW456L13_213 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (Pseudomonas fluorescens GW456-L13)
MSLPSLAELPAILLPFVTRAEQSFRAAVAALDDDHGLSAWTPERWAQFSRVTAASDFVIE
QCVRDPLMLLGLVQSGELDRGFAPGELCGQIAAAANVAETDDQLGRALRRQRARHQVRII
WRDLTRQADLIQTCRDLSDMADASIDQAYQWLYVRHCQQFGVPTGRRSGEPQQMVILGMG
KLGAVELNLSSDIDLIFAYPEGGETVGVKRPLDNQEFFIRLGQRLIKALDPMTVDGFVFR
VDMRLRPYGSAGALVLSFNALEQYYQDQGRDWERYAMIKSRVVAGDQVAGAQLQEMLRPF
VYRRYLDFSAIEALRTMKQLIQQEVRRKGMADNIKLGSGGIREVEFIAQAFQLIHGGRDL
SLQQRPLLKVLSTLEGQGYLPPNVISELREGYEFLRYTEHAIQAIADRQTQMLPDGEQDQ
ARIAFMLGFANWTAFHEQLMYWRGRVAWHFGQVIADPDEEQGAESEVVVGGEWLPLWEEA
QDDEAACRQLEEGGFKDAPKALKALAGLRGSPQLRAMQRLGRERLDAFIPRLLAQAVEHD
NPDLVLERVLPLVEAVARRSAYLVLLTENPSALRRLLTLCAASPWIAEQITRFPLLLDEL
LNEGRLFKPPLAPELAAELRERLTRIPEDDLEQQMEALRHFKLAHRLRVAASEIAGSLPL
MKVSDYLTWLAEAILEQVLALAWRQTVAKYGTPLRTDGTLCDPGFIIVGYGKVGGLELGH
GSDLDLVFIHDGDPQAETDGIKPIDGAQFFTRLGQRIIHLLTAQTNSGQLYEVDMRLRPS
GASGLLVSSLGAFARYQENEAWTWEHQALVRARVLVGSQDVGQAFEKVRAAILGKTRDLP
TLRQEVSEMRAKMRDNLGSKSTAAGTGANAFETTAPFDIKQDAGGIVDIEFMVQYAALAW
SETHPPLLRWTDNIRILEELEHEGLMPVEDASLLREAYKAYRSAAHRQALQKDPGVIPGD
QFADERRQVMRIWRELGLS