Protein Info for PfGW456L13_2016 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): C4-dicarboxylate transporter
Rationale: Specific phenotype: utilization of L-Malic acid, succinate, Tween 20; the phenotype on Tween 20 is not explained
Original annotation: Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 44 to 66 (23 residues), see Phobius details amino acids 78 to 100 (23 residues), see Phobius details amino acids 146 to 169 (24 residues), see Phobius details amino acids 191 to 215 (25 residues), see Phobius details amino acids 221 to 242 (22 residues), see Phobius details amino acids 262 to 280 (19 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details amino acids 328 to 346 (19 residues), see Phobius details amino acids 353 to 375 (23 residues), see Phobius details PF00375: SDF" amino acids 11 to 403 (393 residues), 384.7 bits, see alignment E=2.5e-119

Best Hits

Swiss-Prot: 93% identical to DCTA_PSECL: C4-dicarboxylate transport protein (dctA) from Pseudomonas chlororaphis

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 92% identity to pba:PSEBR_a4274)

MetaCyc: 77% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QNX5 at UniProt or InterPro

Protein Sequence (444 amino acids)

>PfGW456L13_2016 C4-dicarboxylate transporter (Pseudomonas fluorescens GW456-L13)
MTTRQPLYKSLYFQVIVAIAIGILLGHFYPQTGVALKPLGDGFIKLIKMVIAPIIFCTVV
SGIAGMQNMKSVGKTGGYALLYFEIVSTLALLIGLVVVNVVQPGAGMHIDVSTLDTSKIA
GFISAGKDQSIIAFVLNVIPNTIVGAFANGDILQVLMFSVIFGFALHRLGAYGKPVLDFI
DRFAHVMFNIINMIMKLAPVGAFGAMAFTIGAYGVGSLVQLGQLMICFYITCILFVLLVL
GAICRAHGFSVIKLIRYIREELLIVLGTSSSESALPRMLIKLERLGAQKSVVGLVIPTGY
SFNLDGTSIYLTMAAVFIAQATDTPMDLTAQITLLLVLLLSSKGAAGVTGSGFIVLAATL
SAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATLVVAKWVKELDEDTLQVELASGG
RGISDEREVDDLGVAEGPAPASVK