Protein Info for PfGW456L13_1977 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): Succinylglutamate desuccinylase (EC 3.5.1.96)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCGLUDESUCC-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Succinylglutamate desuccinylase (EC 3.5.1.96)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 95% identical to ASTE_PSEPF: Succinylglutamate desuccinylase (astE) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K05526, succinylglutamate desuccinylase [EC: 3.5.1.96] (inferred from 95% identity to pfo:Pfl01_4279)

Predicted SEED Role

"Succinylglutamate desuccinylase (EC 3.5.1.96)" in subsystem Arginine and Ornithine Degradation (EC 3.5.1.96)

MetaCyc Pathways

L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.96

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QIP5 at UniProt or InterPro

Protein Sequence (336 amino acids)

>PfGW456L13_1977 Succinylglutamate desuccinylase (EC 3.5.1.96) (Pseudomonas fluorescens GW456-L13)
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHG
NETAPIELLDRLLHDIARGDLKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSS
GCEALRACELERLAASFFSLPDRQRLHYDLHTAIRGSKIEQFALYPWKEDRQHSRQELAR
LRAAGMEAVLLQNKPSIVFSSYTYDKLGAEAFTLELGKARPFGQNDGVNVSLLEKRLKQI
IEGTEPELTEDALDGLQLYSVAREIIKHSDSFRLNLPADIENFSELEVGYLLAEDIANTR
WIIEEEGARIIFPNPRVKNGLRAGILVVPTTDENLA