Protein Info for PfGW456L13_1974 in Pseudomonas fluorescens GW456-L13
Updated annotation (from data): Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCGLUALDDEHYD-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to ASTD_PSEPF: N-succinylglutamate 5-semialdehyde dehydrogenase (astD) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K06447, succinylglutamic semialdehyde dehydrogenase [EC: 1.2.1.71] (inferred from 95% identity to pfo:Pfl01_4282)MetaCyc: 60% identical to aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Succinylglutamate-semialdehyde dehydrogenase. [EC: 1.2.1.71]
Predicted SEED Role
"Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71)" in subsystem Arginine and Ornithine Degradation (EC 1.2.1.71)
MetaCyc Pathways
- L-arginine degradation II (AST pathway) (5/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.71
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QNT9 at UniProt or InterPro
Protein Sequence (488 amino acids)
>PfGW456L13_1974 Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) (Pseudomonas fluorescens GW456-L13) MKSLYIAGEWLAGGGEAFESLNPVTQQVLWSGVGATAGQVESAVQAARQAFPDWARRTLE ERISVLEAFAAALKNHADELAHTIGEETGKPLWEAATEVTSMVNKIAISVQSYRERTGEK SGPLGDATAVLRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGNSVLFKPSELTPKVAEL TVKCWIEAGLPAGVLNLLQGARETGIALAANPGIDGLFFTGSSRTGNHLHQQFAGRPDKI LALEMGGNNPLVVDQVADLDAAVYTIIQSAFISAGQRCTCARRLLVPQGAWGDSLLARLV AVSSTLSVGAFDQQPAPFMGSVVSLGAAKALMDAQEHLLANGAVALLEMTQPQAQSALLT PGILDVSAVADRPDEELFGPLLQVIRYADFEAAIAEANDTAYGLAAGLLSDSEARYQQFW LESRAGIVNWNKQLTGAASSAPFGGVGASGNHRASAYYAADYCAYPVASLETPSLVLPSA LTPGVKMA