Protein Info for PfGW456L13_1818 in Pseudomonas fluorescens GW456-L13

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 836 transmembrane" amino acids 29 to 51 (23 residues), see Phobius details amino acids 185 to 202 (18 residues), see Phobius details amino acids 222 to 240 (19 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 271 to 386 (116 residues), 70 bits, see alignment E=2e-23 PF00989: PAS" amino acids 272 to 373 (102 residues), 40.3 bits, see alignment E=1e-13 PF08448: PAS_4" amino acids 274 to 381 (108 residues), 46.4 bits, see alignment E=1.5e-15 PF13426: PAS_9" amino acids 279 to 378 (100 residues), 49.1 bits, see alignment E=2.1e-16 PF08447: PAS_3" amino acids 290 to 373 (84 residues), 33.8 bits, see alignment E=1.2e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 388 to 556 (169 residues), 142.4 bits, see alignment E=1.1e-45 PF00990: GGDEF" amino acids 391 to 552 (162 residues), 152.1 bits, see alignment E=4.1e-48 PF00563: EAL" amino acids 574 to 808 (235 residues), 239.2 bits, see alignment E=1.5e-74

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QL50 at UniProt or InterPro

Protein Sequence (836 amino acids)

>PfGW456L13_1818 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens GW456-L13)
MDFFRNKTAAKQAIPTSSNKPRSTKWHRMYFFLAGFDVLVVVLSVLLNHLIVDTYHRSIK
ANQSWVQQLSSYSTLGSLASTVNAPGNNVFDTHNVEAESRNMNEALRAFNEHLAMAKKQL
EVRIANEAEHSADIQAFTLALKDEVGAVDAAMVEMVNEALLIFSYFREGQSDNAGKRMAT
MDRKFALLLASLSTVRDRVGLIQSKLLEEELESVEELRRVEYAIMVFVLLMVSAAVIYGG
KIRREMESQAAEREGYLAVLRESEKQARQQASLLDKAQDAIVVHGMDNRILYWNKSAERL
YGLSKEEALGKSAQELIYQGSAAFNAATDSLIETGDWADEVTLRRRDGCTITLESHRTLV
RDDDGQPQSVLSINTDITHRKTAEQEVQRLAFYDQLTGLANRRLMLDRLQHLLAGSARSL
HTNGIILIDLDNFKALNDTLGHDRGDMLLQQVALRLCGCIRESDTVARLGGDEFVVLLDG
LNENPPEAAIQAKAIGEKILNSLNISYQLDGYEHHSTPSLGIALFQGQLSTADDILKRAD
LAMYRAKAAGRNTMRFFDPEMQAVATARARLEVDLRQGLQDNEFLLHYQPQVNSEGRIIG
AEALMRWQNPERGVVFPGEFIPLAEETGLILPLGRWALQAACTQLVAWAKRSETARLGMA
VNVSARQFHHPDFVEEVLQVLRYTGADPKKLKLELTEGLLIEDMEGTVAKMIELKEIGIG
LSLDDFGTGYSSLSYLKSLPLDQLKIDQSFIRDVLVDPNDAAIACAIVSLSQILGLSVIA
EGVETEAQRIFLVKHGCQTYQGFLFSPPLPAEQFEEYVHERLMASTDFTRTKTKSP