Protein Info for PfGW456L13_1739 in Pseudomonas fluorescens GW456-L13
Annotation: Ornithine carbamoyltransferase (EC 2.1.3.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to OTC_PSESM: Ornithine carbamoyltransferase (argF) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
KEGG orthology group: K00611, ornithine carbamoyltransferase [EC: 2.1.3.3] (inferred from 97% identity to pfo:Pfl01_4527)MetaCyc: 36% identical to ornithine carbamoyltransferase ArgI (Escherichia coli K-12 substr. MG1655)
Ornithine carbamoyltransferase. [EC: 2.1.3.3]
Predicted SEED Role
"Ornithine carbamoyltransferase (EC 2.1.3.3)" in subsystem Arginine Biosynthesis extended or Arginine Deiminase Pathway or Arginine and Ornithine Degradation (EC 2.1.3.3)
MetaCyc Pathways
- superpathway of arginine and polyamine biosynthesis (16/17 steps found)
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
- L-citrulline degradation (3/3 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- urea cycle (3/5 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (2/6 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.3.3
Use Curated BLAST to search for 2.1.3.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QMM1 at UniProt or InterPro
Protein Sequence (307 amino acids)
>PfGW456L13_1739 Ornithine carbamoyltransferase (EC 2.1.3.3) (Pseudomonas fluorescens GW456-L13) MMSARHFLSLMDCTPEELVSVIRRGVELKDLRNRGVLFEPLKNRVLGMIFEKSSTRTRIS FEAGMIQLGGQAIFLSPRDTQLGRGEPIGDCAIVMSSMLDAVMIRTFAHSNLTEFAANSR VPVINGLSDDLHPCQLLADMQTFLEHRGSIQGKTVAWIGDGNNMCNSYIEAAIQFDFQLR VACPEGYEPNPVFLAKAGDRVTIVRDPKEAVRGAHLVSTDVWTSMGQEEETAKRLQLFAP YQVNRALLDLAAEDVLFMHCLPAHRGEEISIDLLDDKRSVAWDQAENRLHAQKALLEFLV EPAYHHA