Protein Info for PfGW456L13_1732 in Pseudomonas fluorescens GW456-L13
Annotation: Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to GLPD_PSEAE: Glycerol-3-phosphate dehydrogenase (glpD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00111, glycerol-3-phosphate dehydrogenase [EC: 1.1.5.3] (inferred from 91% identity to pba:PSEBR_a4407)MetaCyc: 72% identical to aerobic glycerol-3-phosphate dehydrogenase (Pseudomonas putida KT2440)
Glycerol-3-phosphate dehydrogenase. [EC: 1.1.5.3]; 1.1.5.3 [EC: 1.1.5.3]; 1.1.5.3 [EC: 1.1.5.3]
Predicted SEED Role
"Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Respiratory dehydrogenases 1 (EC 1.1.5.3)
MetaCyc Pathways
- glycerol and glycerophosphodiester degradation (4/4 steps found)
- glycerol degradation I (3/3 steps found)
- glycerol-3-phosphate shuttle (2/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- sn-glycerol 3-phosphate anaerobic respiration (2/3 steps found)
- glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer (1/2 steps found)
- glycerol-3-phosphate to fumarate electron transfer (1/2 steps found)
- glycerol-3-phosphate to hydrogen peroxide electron transport (1/2 steps found)
- nitrate reduction IX (dissimilatory) (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.5.3
Use Curated BLAST to search for 1.1.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QUR2 at UniProt or InterPro
Protein Sequence (528 amino acids)
>PfGW456L13_1732 Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) (Pseudomonas fluorescens GW456-L13) MFEFKYRTSKNSEAEPMPTSTLPTPPLAEVYDIAVIGGGINGVGIAADAAGRGLSVFLCE KDDLASHTSSASSKLIHGGLRYLEHYEFRLVREALAEREVLLAKAPHIVKQMRFVLPHRP HLRPAWMIRAGLFLYDNLGKREQLQGSKSLKFGPDSALKSEITKGFEYSDCWVDDARLVV LNAMAAREKGAHVHTQTRCVSARRTKGLWHLHLERADGSLFSIRAKALVNAAGPWVARFI RDDLKMESPYGIRLIQGSHLIVPKLYEGEHAHILQNEDQRIVFTIPYLNHFTLIGTTDRE YTGDPAKVAITDGETDYLLNVVNAHFKKQISREDILHSYSGVRPLCNDESDNPSAVTRDY TLALSGTGEEAPLLSVFGGKLTTYRKLAESALAQLAPYFKHIQPSWTASATLPGGEDMTT PQALSSRIRDKFDWVPSDIARRWATSYGSRTWRMLEGVQDLSDMGEHIGGGLYTREVDYL CSEEWATTAHDILWRRSKLGLFTTAAEQEKLKDYLNKIEQNRNKIEAA