Protein Info for PfGW456L13_1569 in Pseudomonas fluorescens GW456-L13

Annotation: Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00005: ABC_tran" amino acids 31 to 177 (147 residues), 127.6 bits, see alignment E=7.8e-41 TIGR01187: polyamine ABC transporter, ATP-binding protein" amino acids 46 to 370 (325 residues), 372 bits, see alignment E=1.2e-115 PF08402: TOBE_2" amino acids 294 to 370 (77 residues), 54.9 bits, see alignment E=1.2e-18

Best Hits

Swiss-Prot: 84% identical to POTA_PSEF5: Spermidine/putrescine import ATP-binding protein PotA (potA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K11072, spermidine/putrescine transport system ATP-binding protein [EC: 3.6.3.31] (inferred from 84% identity to pfl:PFL_5174)

Predicted SEED Role

"Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.31

Use Curated BLAST to search for 3.6.3.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QHJ3 at UniProt or InterPro

Protein Sequence (372 amino acids)

>PfGW456L13_1569 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) (Pseudomonas fluorescens GW456-L13)
MNALHSLQTLAVSIRSVRKVYGDPKTGPVALKSIDLDIRDNEFFTLLGPSGCGKTTLLRM
IAGFEFPTEGEILLYGENIADRPPFQRPVNTVFQHYALFPHMTIAENLAFGLESHPMGKV
LHKTQLAERVREMLALVQMERFANRKPAQLSGGQQQRVALARALAPHPKVLLLDEPLSAL
DLKLRQAMREELKTIQARTGITFIFVTHDQEEALTMSDRIAVLSEGEVQQVGRPEDIYER
PRNRFVADFIGETNFIEGTVTRVEDGLAWFAGPAGHPLPAQPCSDVRVGANVTLSVRPER
LHLVPATTENALPCRIEAQIYLGTDLQYQVSLSDGSRLTVRTPNCVDQSKRFAVGSQAGL
LFDQGSASVLHD