Protein Info for PfGW456L13_1535 in Pseudomonas fluorescens GW456-L13

Annotation: Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 739 TIGR02071: penicillin-binding protein 1B" amino acids 1 to 715 (715 residues), 1005.1 bits, see alignment E=8.6e-307 PF17757: UvrB_inter" amino acids 34 to 106 (73 residues), 31.1 bits, see alignment E=4.1e-11 PF14814: UB2H" amino acids 36 to 119 (84 residues), 84.3 bits, see alignment E=1e-27 PF00912: Transgly" amino acids 133 to 301 (169 residues), 197.4 bits, see alignment E=2.8e-62 PF00905: Transpeptidase" amino acids 396 to 635 (240 residues), 62.4 bits, see alignment E=8.7e-21

Best Hits

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 95% identity to pba:PSEBR_a4798)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QHF8 at UniProt or InterPro

Protein Sequence (739 amino acids)

>PfGW456L13_1535 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) (Pseudomonas fluorescens GW456-L13)
VVLAGFAVYLDAIVQEKFSGKRWTIPAKVYARPLELFVGQKLSRDDFLTELDALGYRRES
VANGPGAASVNGNTVDLNTRGFQFYEGMEQAQPVRVRFSGDYVAELAGAKGSKLSVVRLE
PLLIGGLYPKNLEDRILIKIDQVPPYLLETLVAVEDRDFYHHFGVSPKSIARAIWVNGSS
GRMRQGGSTLTQQLVKNFYLTNERSLSRKLTEAMMALLLELHYDKREILEAYLNEVFVGQ
DGQRAVHGFGLASQFFFSQPLAELKLHQVALLVGMVKGPSSYNPRRNPERALERRNLVLD
VLQEQGVATAEQVEAAKKMPLGVTKRGSLADSSFPGFLDLVKRQLREDYRDEDLTEEGLR
IFTSFDPILQMKAEASVNDTFKRLAGRKGSDDVEAAMVVTNPETGEVQAMIGSRQASFAG
FNRALDAVRPIGSLIKPAVYLTALEKPSQYTLTSWLSDESFSVKGADGQVWKPQNYDRRS
HGTVFLYQGLAHSYNLSTARLGLAVGVPNVLKTVARLGVTREFPAFPSMLLGAGGMTPIE
VATMYQTLANGGFNTPMRGIRSVLTAEGEPLKRYPFQIQQRFDPASIYLIQSAMQRVMRE
GTGSSVYNALPRTLTLAGKTGTSNDSRDSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGA
LQVWTSFMRKADPLPLDMPQPDNVVQAWVDSRTGQGSDAGCPGAVQMPYIRGSEPPPGAA
CGGENPAETVMDWVKGWMN