Protein Info for PfGW456L13_1475 in Pseudomonas fluorescens GW456-L13

Annotation: Proposed peptidoglycan lipid II flippase MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details amino acids 48 to 75 (28 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 126 to 146 (21 residues), see Phobius details amino acids 153 to 170 (18 residues), see Phobius details amino acids 201 to 227 (27 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details amino acids 275 to 297 (23 residues), see Phobius details amino acids 319 to 339 (21 residues), see Phobius details amino acids 348 to 367 (20 residues), see Phobius details amino acids 373 to 392 (20 residues), see Phobius details amino acids 413 to 431 (19 residues), see Phobius details amino acids 443 to 466 (24 residues), see Phobius details PF03023: MurJ" amino acids 1 to 442 (442 residues), 508.2 bits, see alignment E=1.9e-156 TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 2 to 467 (466 residues), 488.7 bits, see alignment E=1e-150 PF14667: Polysacc_synt_C" amino acids 318 to 465 (148 residues), 45.2 bits, see alignment E=1.1e-15

Best Hits

Swiss-Prot: 73% identical to MURJ_SALTY: Probable lipid II flippase MurJ (murJ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03980, virulence factor (inferred from 95% identity to pfo:Pfl01_4854)

MetaCyc: 73% identical to lipid II flippase MurJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-286

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QMB4 at UniProt or InterPro

Protein Sequence (477 amino acids)

>PfGW456L13_1475 Proposed peptidoglycan lipid II flippase MurJ (Pseudomonas fluorescens GW456-L13)
MATDAFFIAFKLPNLLRRIFAEGAFSQAFVPILAEYKSQKGDEATRTFIAYVSGLLTLVL
ALVTALGMIAAPWVIWVTAPGFIDTPEKFELTTSLLRVTFPYILLISLSSLAGAILNTWN
RFSVPAFVPTLLNVSMIIFALFLTPYFDPPVMALGWAVLVGGLAQLLYQLPHLKKIGMLV
LPRLNLRDSGVWRVMKQMLPAILGVSVSQISLIINTIFASFLVAGSVSWMYYADRLMELP
SGVLGVALGTILLPTLAKTYASKDRHEYSRILDWGLRLCFVLVLPCAFALGILAEPLTVS
LFQYGQFDAHDAAMTQRALIAYSVGLLGIIVIKVLAPGFYAQQNIRTPVKIAIFTLVVTQ
LFNLLLIGPLAHAGLALAISAGACLNAGLLFYQLRKQQMYQPQPGWAKFGFKLVVAVAVM
SGVLLAGMHFMPAWGEGQMLERFLRLGALVVAGVVAYFGMLVLMGFRLRDFNRKALG