Protein Info for PfGW456L13_1472 in Pseudomonas fluorescens GW456-L13

Annotation: Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 6 to 366 (361 residues), 433.8 bits, E=2.7e-134
• PF00696: AA_kinase
  • amino acids 6 to 234 (229 residues), 164.6 bits, E=3.3e-52
• PF01472: PUA
  • amino acids 277 to 347 (71 residues), 74.7 bits, E=4.6e-25

Best Hits

Swiss-Prot: 98% identical to PROB_PSEPF: Glutamate 5-kinase (proB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 97% identity to pfl:PFL_5326)

MetaCyc: 44% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Sequence Analysis Tools

See A0A293QK60 at UniProt or InterPro

Protein Sequence (368 amino acids)

>PfGW456L13_1472 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA (Pseudomonas fluorescens GW456-L13)
VTGAQRWVVKIGSALLTADGKGLDRAAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRL
GWTSRPSAMHELQAAAAIGQMGLVQAWESSFAKHGRHTAQILLTHDDLSDRKRYLNARST
LRALVELKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFDADPR
NNPDAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVGGRLER
VLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDAGAVTALSQGNKSLLPVG
VKLVQGSFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQSSEAIVGLLGYMAEPELV
HRDNLILV