Protein Info for PfGW456L13_1452 in Pseudomonas fluorescens GW456-L13

Annotation: Serine hydroxymethyltransferase (EC 2.1.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00229: PAS domain S-box protein
  • amino acids 425 to 557 (133 residues), 46 bits, E=5.3e-16
  • amino acids 558 to 697 (140 residues), 44.7 bits, E=1.4e-15
  • amino acids 702 to 818 (117 residues), 67.3 bits, E=1.4e-22
• PF13426: PAS_9
  • amino acids 449 to 549 (101 residues), 22.1 bits, E=5.2e-08
  • amino acids 574 to 689 (116 residues), 27.1 bits, E=1.4e-09
  • amino acids 709 to 811 (103 residues), 62.5 bits, E=1.4e-20
• PF08447: PAS_3
  • amino acids 453 to 544 (92 residues), 43 bits, 1.6e-14
• PF00989: PAS
  • amino acids 702 to 809 (108 residues), 39.6 bits, E=1.6e-13
• PF08448: PAS_4
  • amino acids 704 to 813 (110 residues), 34.1 bits, E=9.9e-12
• TIGR00254: diguanylate cyclase (GGDEF) domain
  • amino acids 820 to 986 (167 residues), 137.7 bits, E=3.1e-44
• PF00990: GGDEF
  • amino acids 823 to 984 (162 residues), 168.1 bits, E=5.1e-53
• PF00563: EAL
  • amino acids 1004 to 1239 (236 residues), 263.6 bits, E=5.3e-82

Best Hits

KEGG orthology group: None (inferred from 90% identity to pfo:Pfl01_4876)

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• glycine betaine degradation III (7/7 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• glycine betaine degradation I (6/8 steps found)
• folate transformations I (9/13 steps found)
• photorespiration I (6/9 steps found)
• photorespiration II (6/10 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• photorespiration III (5/9 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.1

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See A0A293QH88 at UniProt or InterPro

Protein Sequence (1258 amino acids)

>PfGW456L13_1452 Serine hydroxymethyltransferase (EC 2.1.2.1) (Pseudomonas fluorescens GW456-L13)
LKGTLATLVLLLLALLFWQLLDQLRETQKNQRQYTIDYTADLAAQVSLNMALNAQIALNL
LPIVEQPQGADELQALLRKLQQSLPDLRSLALLSPSGRVISDSAPDSDDNNYLGELVRRS
HAQAHYFSNATDGSVVHLLLHQASGSSRGYWVLRLTPTFFSSLTKQREAGGRPLWLVENR
ISHQIISRDEALPSVNPGTLTEDDVANSVLTVPLSSSDWQLRGLFDRQRVLEELLPAFIG
KCLLGLAFSMLPFLALLNTRRRQRQLNEGRRRYQDIFEGTGVALCVLDMSGLKVFFDKAQ
LQGSEQLQAWLAIPEQRRPLLKELRITEVNQVALQLLNVDSADQAWKLLIDGNPQDGTAI
GNQVLDAMLNQQQQLELEIKLQDASGRIQHLWLVLCLPQTPDDYNAVILSITDITSRKLI
ELSLQEREGFWSDVVRTVPDHLYVQDVISQRMIYSNHHLGQTLGYNRNELHQMGEYFWEI
LLHPEDADYYHRSRQIQRQAGYRQLMQCQLRFRHRDGQWRRFEIREQALARDKDDQVTRI
IGVAKDITDQIEASESLRDSEQRYRMLAESISDVIFSTDSRLNLNYVSPSVLAVLGYDAE
WIFQNGWQSTIANPQQLTGIYHLMDRVSKALDQPDQLALLRSQVQTQLFLFDCLRADGRK
IPIELRLVLVWDEYGTFEGVLGVGRDISQQRRAEKDLRMAATVFEHSTSAILITDPAGYI
VQANEAFSRVSGYAVEQVLDQLPNMLTVDDQQEAHLRYVLKQLGQHSTWEGEVWLKRRNG
EHYPAWVGITAVLDDEGDLASYVCFFSDISERKASEQRIHRLAYYDALTHLPNRSLFQDR
LHTALQSAERQKSWVVLMFLDLDRFKPINDSLGHAAGDRMLKEMATRLLGCVDEDDTVAR
MGGDEFTLLLQPRANREIALNRAIHVAEQILASLVKPFVLEGREFFVTASIGIALSPQDG
NELSQLMKNADTAMYHAKERGKNNFQFYQADMNASALERLELESDLRHALEQNEFVLYYQ
PQFSGDGKRLTGAEALLRWRHPRRGLVPPGDFIPVLEELGLVVDVGDWVISEACRQLKAW
HQARVRVPKVSVNISARQFSDGQLGTRIATILKETGLPPACLELELTESILMREVSEAMQ
ILDGLKNLGLSIAVDDFGTGYSSLNYLKQFPIDVLKIDRTFVDGLPSGEQDAQIARAIIA
MAHSLNLAVIAEGVETHEQLDFLREHGCDEVQGYLFGRPMPAGRFEGQFSNDALFMLD