Protein Info for PfGW456L13_1418 in Pseudomonas fluorescens GW456-L13

Annotation: 3-oxosteroid 1-dehydrogenase (EC 1.3.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 transmembrane" amino acids 20 to 31 (12 residues), see Phobius details amino acids 165 to 183 (19 residues), see Phobius details PF01946: Thi4" amino acids 11 to 57 (47 residues), 30.8 bits, see alignment 7.2e-11 PF01266: DAO" amino acids 14 to 303 (290 residues), 66.7 bits, see alignment E=1.1e-21 PF00890: FAD_binding_2" amino acids 14 to 552 (539 residues), 359.8 bits, see alignment E=9.9e-111 PF01134: GIDA" amino acids 14 to 64 (51 residues), 19.2 bits, see alignment 2.2e-07 PF12831: FAD_oxidored" amino acids 14 to 57 (44 residues), 37.2 bits, see alignment 9.6e-13 PF03486: HI0933_like" amino acids 14 to 56 (43 residues), 25 bits, see alignment 3.1e-09 PF13450: NAD_binding_8" amino acids 17 to 51 (35 residues), 28.1 bits, see alignment (E = 7.8e-10)

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_4911)

Predicted SEED Role

"3-oxosteroid 1-dehydrogenase (EC 1.3.99.4)" (EC 1.3.99.4)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.4

Use Curated BLAST to search for 1.3.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QH59 at UniProt or InterPro

Protein Sequence (582 amino acids)

>PfGW456L13_1418 3-oxosteroid 1-dehydrogenase (EC 1.3.99.4) (Pseudomonas fluorescens GW456-L13)
MPAQAHFLPDIDCDVLIVGSGAAGLSAAVTAAWHGLKVIVVEKDPVFGGATAWSGGWAWV
PCNPLARRAGIVEDVELPRTYLKHELGERFDPAMIDAFLEAGPRMVSFFEQYTSLQFADG
NAIADIHGDTPGAGTGGRSVIAAPYDARKVGTLLKRLRKTMRETSFMGMPIMAGADLTAF
LNLTRSLSAAWHVTRRFSRHLFDLVVHGRAMQLVNGVALVARLAKSADDLGVLLWESAPV
SRLLRENDQVRGAVVNTAKGSVRIHARKAVVLAAGGFANDIDRRKALFPRTPTGHEHLAL
PPLGVCGDGLRLGESAGARVNTDMASAVAWAPVSQVPHSDGSTGHFPHIIERGKPGIIGV
LSNGQRFVNEANGYYDYVTAMVAAAPPGEEVASWLICTHGFQRRYGLGISRPFPVPLSSF
IRSGYLKTGNTLEELAAACGIDPVGLRTTVSDYNRHARNGEDPQFGRGSTPYNRKQGDAL
QQPNPCVAPIEQGPFYAVKVQPGCFGTFAGLKVNHHAQVLDGAAQPIAGLYAAGGDMASI
MGGHYPAGGINLGPALTFGYIAARHIAGVTAYEKEIDHAAHH