Protein Info for PfGW456L13_1297 in Pseudomonas fluorescens GW456-L13

Annotation: Putative drug efflux protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 40 to 62 (23 residues), see Phobius details amino acids 69 to 88 (20 residues), see Phobius details amino acids 95 to 117 (23 residues), see Phobius details amino acids 128 to 149 (22 residues), see Phobius details amino acids 160 to 179 (20 residues), see Phobius details amino acids 203 to 227 (25 residues), see Phobius details amino acids 233 to 255 (23 residues), see Phobius details amino acids 267 to 286 (20 residues), see Phobius details amino acids 291 to 315 (25 residues), see Phobius details amino acids 328 to 355 (28 residues), see Phobius details amino acids 358 to 378 (21 residues), see Phobius details PF07690: MFS_1" amino acids 8 to 322 (315 residues), 166.3 bits, see alignment E=1.4e-52 PF06779: MFS_4" amino acids 12 to 375 (364 residues), 53.7 bits, see alignment E=3.3e-18 PF00083: Sugar_tr" amino acids 40 to 175 (136 residues), 28.1 bits, see alignment E=1.5e-10

Best Hits

Swiss-Prot: 56% identical to YTBD_BACSU: Uncharacterized MFS-type transporter YtbD (ytbD) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 96% identity to pfo:Pfl01_5034)

Predicted SEED Role

"Putative drug efflux protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QKL5 at UniProt or InterPro

Protein Sequence (403 amino acids)

>PfGW456L13_1297 Putative drug efflux protein (Pseudomonas fluorescens GW456-L13)
MPIALLALTLSAFAIGTTEFVIVGLLPTIGADLGVSLPSAGLLVSLYALGVAVGAPVLTA
LTGKVPRKLLLLSLMVLFTLGNLLAWQAPSYESLILARIVTGLAHGVFFSIGSTIATSLV
PKEKAASAIAIMFTGLTVALVTGVPLGTFIGQHFGWRETFLAVSALGVIAFIGSLVYVPK
NIAHSKPASLLQQLQVLKQPRLLLVYAMTAVGYGGSFIAFTFLAPILQDLSGFSASTVSL
VLLVYGISVAIGNIWGGKLADKRGPISALKIIFALLAAVLFVLTFTAGNPWLALATVLVW
GAVAFGNVPGLQVYVVRQAEHHTPQAVDVASGLNIAAFNLGIAGGAWGGGLIVAHMGLIH
TAWIGGLVVLVALALTAWSGRLDRLGPVYAEPAEGSARVITAH