Protein Info for PfGW456L13_1193 in Pseudomonas fluorescens GW456-L13

Annotation: tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 PF01743: PolyA_pol" amino acids 4 to 122 (119 residues), 65 bits, see alignment E=1.3e-21 PF12627: PolyA_pol_RNAbd" amino acids 149 to 212 (64 residues), 47.3 bits, see alignment E=2.2e-16 PF01966: HD" amino acids 230 to 316 (87 residues), 41 bits, see alignment E=3.2e-14

Best Hits

Swiss-Prot: 91% identical to CCA_PSEPF: Multifunctional CCA protein (cca) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 91% identity to pfo:Pfl01_5142)

MetaCyc: 58% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QIW4 at UniProt or InterPro

Protein Sequence (409 amino acids)

>PfGW456L13_1193 tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25) (Pseudomonas fluorescens GW456-L13)
MRIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYAL
ARTERKSGRGYGGFTFHASPEVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEARL
LRHVSPAFAEDPLRVLRVARFAARYATLGFTVAPETLALMHQLSESGELEALTPERSWKE
ISRALLEDQPQVFVEVLRACGALKVLMPEVDALFGIPQPPAHHPEIDTGAHTLSVLQQAA
LHKQPLTVRWACLLHDLGKGLTPEDEWPRHIAHEFKGLKLIKAVNERFKVPRDCQELALL
VGEYHTHGHRALELKPSTLLELLQSFDIYRRPQRFEEFIAACEMDARGRKGLEERSYPQA
DYLRGAATAARSVAVQPLLEKGFKGPELGEEIKRERLKALKAYKEAAST