Protein Info for PfGW456L13_1173 in Pseudomonas fluorescens GW456-L13

Annotation: Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 784 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 42 to 59 (18 residues), see Phobius details amino acids 66 to 82 (17 residues), see Phobius details amino acids 88 to 112 (25 residues), see Phobius details amino acids 124 to 144 (21 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 17 to 783 (767 residues), 1119.2 bits, see alignment E=0 PF13360: PQQ_2" amino acids 198 to 372 (175 residues), 37.8 bits, see alignment E=2.6e-13 amino acids 284 to 455 (172 residues), 24.5 bits, see alignment E=3e-09 PF01011: PQQ" amino acids 225 to 258 (34 residues), 29.7 bits, see alignment (E = 5.7e-11) amino acids 285 to 317 (33 residues), 21.4 bits, see alignment (E = 2.4e-08) amino acids 438 to 463 (26 residues), 26.1 bits, see alignment (E = 7.6e-10)

Best Hits

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 91% identity to pfl:PFL_5668)

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QLH8 at UniProt or InterPro

Protein Sequence (784 amino acids)

>PfGW456L13_1173 Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25) (Pseudomonas fluorescens GW456-L13)
MNNSGAAAGSKWLLAGLGVLIALIGLGLAGGGGYLIALGGSWYFLLMGLAMLASGLMIAR
RKPLGAWLYGVALVLTAIWAVWDAGLEYWPLVSRILTFAVIGLVVALIYPTLVRASGATG
GRGAYGLAGILGVAVVATMAYMFVPTHVVKATTEPAVTPVTPGTEQKDWAHWGNTTAGNR
FAALDQINKGNIDKLQVAWTFRTGDIPQSTGAGAEDQNTPLQIGDTVYTCTAYGKVFALD
ADTGAQRWKFDPQGTAPNWQRCRGLGYSETPVSSDNQPTACTKRLFLPTGDARLIAINAD
TGKPCEEFGNKGTVDLTTDMGEVKPGYYQQTSTPLVAGDVVIVGGRVADNYSTGEPPGVV
RAYDVRSGELVWAWDPGNPNTTKRPPAGETYTRGTPNVWSAMSYDAKLGLVYLPTGNATP
DFFGGQRTEFDDKWSSSIVAIDVKTGQVRWHFQTTHHDLWDFDLPAQPLLYDVPDDKGGT
QPALAQVTKQGEIFLLNRETGVPIARVEERPVPQGKVPGERYSPTQPFSVDMPSIGNKTL
TESDMWGATPFDQLMCRIQFKGMRHEGVYTPPGMDRALQFPGSLGGMNWGSVSVDPNTHY
MFINDMRLGLANYMIPRDKIGAGASGIEMGVVPQEGTPFGAMRERFLSAVGIPCQKPPFG
TMSAIDLKTHKLVWQVPVGTVQDTGPLGIRMHLPIPIGMPTLGASLATQSGLLFFAGTQD
FYLRAFDTGNGNEIWKSRLPVGSQSGPMTYVSPKTGRQYILLTVGGARQSTDRGDYVIAY
ALPK