Protein Info for PagCFBP13505_RS12250 in Pantoea agglomerans CFBP13505 P0401

Annotation: bifunctional UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronic acid oxidase ArnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 659 PF00551: Formyl_trans_N" amino acids 42 to 176 (135 residues), 91 bits, see alignment E=1.6e-29 PF02911: Formyl_trans_C" amino acids 202 to 295 (94 residues), 89.1 bits, see alignment E=3.7e-29 PF01370: Epimerase" amino acids 318 to 566 (249 residues), 147.8 bits, see alignment E=7.5e-47 PF16363: GDP_Man_Dehyd" amino acids 319 to 644 (326 residues), 92.1 bits, see alignment E=1e-29

Best Hits

Swiss-Prot: 79% identical to ARNA_KLEP3: Bifunctional polymyxin resistance protein ArnA (arnA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K10011, UDP-4-amino-4-deoxy-L-arabinose formyltransferase / UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) [EC: 1.1.1.305 2.1.2.13] (inferred from 97% identity to pva:Pvag_pPag10070)

MetaCyc: 76% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

"UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305) / UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13)" (EC 1.1.1.305, EC 2.1.2.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (659 amino acids)

>PagCFBP13505_RS12250 bifunctional UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronic acid oxidase ArnA (Pantoea agglomerans CFBP13505 P0401)
MKTVVFAYHDMGCVGINALLSAGYEITAIFTHNDVATENHFFGSVARLAAEHGIPVYAPD
EANHPIWLDRIRTMAPEMIFSFYYRHLLSDEILQCAPKGAFNLHGSLLPKYRGRAPLNWA
LVNGETETGVTLHRMVKRADAGNILAQQKVAIEDQDNALTLHRKLTQAAEQLLGDVLPRL
REGEVSEWPQDESQATRVDRRTAEDGRIDWNQPAQTIHNLVRAVTDPWPGAFAFAGSVKF
IVWKSRVHPQQHQAKPGTVLSKQPFLIACGEGALEIQTGQSEHGVYMNGSQLAQSLGIVP
GALLYSKPQSALQRRTRVLILGVNGFIGNHLTERLLQDEHFEVYGLDISSDAISRFLDHP
RFHFVEGDIAIHSEWIEYHIKKCDVILPLVAIATPIEYTRNPLRVFELDFEENLKIIRDC
VKYQKRIIFPSTSEVYGMCTDDSFDEDCSNLVVGPINKQRWIYSVSKQLLDRVIWAYGEK
EGLRFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGKQKRCFTDI
QDGVEALFRIIENKQLNCDGQIINIGNPDNEASIKELAEQLLASFERHPLRSQFPPFAGF
REVESSSYYGKGYQDVEHRKPAIRNARRLLGWTPTVTMDVTINNTLDFFLRTVEPQADK