Protein Info for PP_5604 in Pseudomonas putida KT2440

Annotation: putative subtilisin-like serine protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 225 PF00082: Peptidase_S8" amino acids 2 to 143 (142 residues), 21.8 bits, see alignment E=5.3e-09

Best Hits

KEGG orthology group: None (inferred from 93% identity to ppf:Pput_2303)

Predicted SEED Role

"Extracellular protease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A140FWF5 at UniProt or InterPro

Protein Sequence (225 amino acids)

>PP_5604 putative subtilisin-like serine protease (Pseudomonas putida KT2440)
MGTDVRVGIIDSGCSPEQANGLLDARRFWLEDGQLREGEMLPDQLGHGSAVLASLQHQAG
PVSVLVAQVFSAQASTSALQVAAALLWLVESGATLVNLSLGLQQDRPVLHQACAEALAAG
VLLCASSPAQGGPVFPASYPGVIRVTGDARCAPGQWSWLGTRQADFGAYVGAGERAGASL
GCAALSGKIAALLRDAQGMDRQQVHDWLREHAAFKGPERRGARHG