Protein Info for PP_5411 in Pseudomonas putida KT2440

Annotation: N-acetyl glucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 451 (448 residues), 652.6 bits, E=1.6e-200
• PF01128: IspD
  • amino acids 5 to 131 (127 residues), 33.3 bits, E=1.3e-11
• PF00483: NTP_transferase
  • amino acids 6 to 165 (160 residues), 52 bits, E=2.4e-17
• PF12804: NTP_transf_3
  • amino acids 6 to 122 (117 residues), 79.3 bits, E=1.2e-25
• PF02348: CTP_transf_3
  • amino acids 22 to 125 (104 residues), 25.9 bits, E=2.4e-09
• PF00132: Hexapep
  • amino acids 263 to 297 (35 residues), 30.4 bits, 6.6e-11
• PF14602: Hexapep_2
  • amino acids 281 to 310 (30 residues), 19.2 bits, see alignment (E = 2.5e-07)
  • amino acids 394 to 426 (33 residues), 20 bits, see alignment (E = 1.4e-07)

Best Hits

Swiss-Prot: 100% identical to GLMU_PSEPK: Bifunctional protein GlmU (glmU) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to ppf:Pput_5293)

MetaCyc: 56% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (10/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See Q88BX6 at UniProt or InterPro

Protein Sequence (455 amino acids)

>PP_5411 N-acetyl glucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyl transferase (Pseudomonas putida KT2440)
MSLDIVILAAGQGTRMRSALPKVLHPVAGNSMLGHVIHSARQLQPQGIHVVIGHGAELVR
ERLAADDLNFVMQDKQLGTGHAVAQALPALTADTVLVLYGDVPLIEVETLQRLLAKANDQ
QLGLLTVTLDDPTGYGRIVRDEQGKVTAIVEHKDANDAQKAIKEGNTGILALPAARLADW
MGRLSNNNAQGEYYLTDVIAMAVADGLVVATEQPHDAMEVQGANDRRQLSELERHYQLRE
GRRLMAQGVTLRDPARFDVRGEVSVGRDVLIDINVILEGKVVIEDDVQIGPNCVIKNTTL
RKGAVVKANSHLEGAVMGEGSDAGPFARLRPGSVLDAKAHVGNFVELKNAHLGEGAKAGH
LTYLGDAEIGARTNIGAGTITCNYDGANKFKTVMGEDVFIGSNNSLVAPVEIKAGATTAA
GSTITQAVEAGDLAVARARQRNISGWKRPEKIKKS