Protein Info for PP_5384 in Pseudomonas putida KT2440

Annotation: copper sensor protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 164 to 184 (21 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 6 to 457 (452 residues), 465 bits, see alignment E=1.5e-143 PF21085: CusS" amino acids 6 to 157 (152 residues), 60.8 bits, see alignment E=3.6e-20 PF00672: HAMP" amino acids 182 to 235 (54 residues), 34.8 bits, see alignment 3.4e-12 PF00512: HisKA" amino acids 240 to 305 (66 residues), 49.2 bits, see alignment E=9.2e-17 PF02518: HATPase_c" amino acids 348 to 458 (111 residues), 81.4 bits, see alignment E=1.3e-26

Best Hits

Swiss-Prot: 53% identical to COPS_PSEUB: Sensor protein CopS (copS) from Pseudomonas syringae pv. tomato

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to ppu:PP_5384)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88BZ9 at UniProt or InterPro

Protein Sequence (469 amino acids)

>PP_5384 copper sensor protein (Pseudomonas putida KT2440)
MILTRSSLVKRLTLMIMFAVIAVLVVAGISFNMLSQHHFRMLDEQALSEKLESTRHILSI
QAPGTGMEELRPQLRALLGAHQDLTAEILTSEGEVLFSDLKAVQIPDKYKRADKEEMWEW
QDESHNFRGITTDVEIQGQPAPGTVMLMLDITSHAHFFETLQRWFAIGLVISALVSAGIG
WLVAKSGLRPVEQITKVATSISARSLQERIPLEPVPLELQTLILSFNGMLARLEDAFVRL
SNFSADIAHELRTPVSNLLTHTEVVLTRKRDVDAYEENLYSNLEDLKRMSRMIDDMLFLA
KSDNGLIIPEQVDIQLHDLVSKLFEYYQLLADDRGIRLTLQGKGVISGDRLMIDRAVSNI
LSNAVRYTPDGSGISVEIQQAADKVTLTIKNGGATIDPQHINKIFDRFYRADPARREGGP
SNAGLGLAITRSIVKAHDGKVWCTSSEGVTAFNFIFPAAHRTAHIRTRH