Protein Info for PP_5365 in Pseudomonas putida KT2440

Annotation: putative fatty acid methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 PF02353: CMAS" amino acids 132 to 406 (275 residues), 380.6 bits, see alignment E=1.3e-117 PF13489: Methyltransf_23" amino acids 189 to 303 (115 residues), 29.6 bits, see alignment E=1.7e-10 PF13847: Methyltransf_31" amino acids 192 to 295 (104 residues), 39.2 bits, see alignment E=1.7e-13 PF08241: Methyltransf_11" amino acids 198 to 295 (98 residues), 52 bits, see alignment E=2.8e-17 PF13649: Methyltransf_25" amino acids 198 to 293 (96 residues), 63 bits, see alignment E=1e-20 PF08242: Methyltransf_12" amino acids 198 to 294 (97 residues), 41.7 bits, see alignment E=4.9e-14

Best Hits

Swiss-Prot: 96% identical to FAMT_PSEPU: Probable fatty acid methyltransferase from Pseudomonas putida

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 100% identity to ppu:PP_5365)

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79)" (EC 2.1.1.79)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C18 at UniProt or InterPro

Protein Sequence (428 amino acids)

>PP_5365 putative fatty acid methyltransferase (Pseudomonas putida KT2440)
MVLLRGNILQMNYSDPVTFEILGVVANYGQEGCFMLAQLPPALQSLHLPLRLKLWDGNHF
DLGPSPQVTILVKEPQLISQLTHPSMDQLGTAFVEGKLELEGDIGEAIRVCDELSEALLA
DEDETPPPRVSHDKTTDAKAISYHYDVSNAFYQLWLDQDMAYSCAYFREPDNTLDQAQQD
KFDHLCRKLRLDAGDYLLDVGCGWGGLARFAAREYGAKVFGITLSKEQLKLGRKRVQAEG
LADKVDLQILDYRDLPQDGRFDKVVSVGMFEHVGHANLALYCQKLFGAVREGGLVMNHGI
TAKHVDGRPVGRGAGEFIDRYVFPHGELPHLSMISASICEAGLEVVDVESLRLHYAKTLH
HWSENLENQLHKAAALVPEKTLRIWRLYLAGCAYAFQKGWINLHQILAVKPYADGHHDLP
WTREDLYR