Protein Info for PP_5364 in Pseudomonas putida KT2440

Annotation: Cardiolipin synthase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 transmembrane" amino acids 10 to 33 (24 residues), see Phobius details amino acids 39 to 66 (28 residues), see Phobius details TIGR04265: cardiolipin synthase" amino acids 19 to 481 (463 residues), 376.1 bits, see alignment E=1.5e-116 PF13396: PLDc_N" amino acids 21 to 61 (41 residues), 23.8 bits, see alignment 5.3e-09 PF13091: PLDc_2" amino acids 138 to 248 (111 residues), 27.4 bits, see alignment E=3.9e-10 amino acids 322 to 447 (126 residues), 100.3 bits, see alignment E=1.2e-32 PF00614: PLDc" amino acids 221 to 247 (27 residues), 25.1 bits, see alignment (E = 2e-09) amino acids 395 to 421 (27 residues), 34.5 bits, see alignment (E = 2.2e-12)

Best Hits

Swiss-Prot: 100% identical to CLSA_PSEPK: Cardiolipin synthase A (clsA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K06131, cardiolipin synthase [EC: 2.7.8.-] (inferred from 100% identity to ppu:PP_5364)

Predicted SEED Role

"Cardiolipin synthetase (EC 2.7.8.-)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.8.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.8.-

Use Curated BLAST to search for 2.7.8.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C19 at UniProt or InterPro

Protein Sequence (481 amino acids)

>PP_5364 Cardiolipin synthase 1 (Pseudomonas putida KT2440)
MHMDYHSPYFFGYLLGLIHLLGIVAALHALFTVRTAQGAIAWAMPLLFIPYLTLIPYLIF
GARSFYAYIKARRQANQEMHVAMANLNWRPWVEEALTARESESYTALRAMPKLGRMPCLA
NNQVKLLINGKATFDAIFAAIEKARDVVLVQFFIIHDDTLGKALQQLLLRKAAEGVQVFV
LYDRVGSHALPSSYSQVLRDGGVQIHAFATRRGWFNRFQVNFRNHRKIVVVDGLLGFIGG
HNVGDEYLGEHPQLSPWRDTHVQISGPVLACLQESFAEDWYWATRQLPPLILPDTYPDNG
VLCQALASGPADPQETCSLFFLEAIHSATRRVWITSPYFIPDEAVFAALRLAVLRGVDVR
VLIPSRPDHRIVYAASSLFAFEAVRAGVRMFRYQPGFLHQKVVLVDDDVSAIGSANLDNR
SFRLNFEITLLTVDRGFADQVEHMLQEDFEQAREITAEDTQDTHRLQQLGMRIARLISPI
L