Protein Info for PP_5353 in Pseudomonas putida KT2440

Annotation: conserved exported protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 93 to 113 (21 residues), see Phobius details PF04280: Tim44" amino acids 159 to 281 (123 residues), 36.8 bits, see alignment E=2.2e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5353)

Predicted SEED Role

"putative membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C30 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PP_5353 conserved exported protein of unknown function (Pseudomonas putida KT2440)
MQRFLSIALALCVGLTLSLDASAKRFGGGKSSGSAPIHQTRQATPTTPAAAPTVPGRAPA
AASGASRWLGPLAGLAAGGLLASMFMGDGFEGFQIMDFLIVALIAFLVFRFIAARRRQQQ
PQMAMPGHAPMQREAHAQPAQPSIFGGSAAPAATAAPVINAPAWFNEQSFLAAARNHFQS
LQQHWDANEMDKISEFVTPQMLEFLKRERAELGDGFQSTYIDNLEVQLDGVDDRADRTDA
TLTFRGVSKNSRFDQGEVFSESWHMVRAQGENQPWLVAGIRQNS