Protein Info for PP_5338 in Pseudomonas putida KT2440

Annotation: aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00839: aspartate ammonia-lyase
  • amino acids 12 to 472 (461 residues), 693.1 bits, E=1e-212
• PF00206: Lyase_1
  • amino acids 19 to 351 (333 residues), 329.3 bits, E=2.9e-102
• PF10415: FumaraseC_C
  • amino acids 417 to 469 (53 residues), 73.5 bits, 1.5e-24

Best Hits

Swiss-Prot: 93% identical to ASPA_PSEFL: Aspartate ammonia-lyase (aspA) from Pseudomonas fluorescens

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 100% identity to ppf:Pput_5247)

MetaCyc: 57% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

• L-glutamate degradation II (2/2 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)
• L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.1

Sequence Analysis Tools

See Q88C45 at UniProt or InterPro

Protein Sequence (478 amino acids)

>PP_5338 aspartate ammonia-lyase (Pseudomonas putida KT2440)
MIYIMSSAASFRVEKDLLGTLEVPADAYYGIQTLRAANNFHLSGVPLSHYPKLVVALAMV
KQAAADANRELGHLSDAKHAAISAACARLIKGDFHDQFVVDMIQGGAGTSTNMNANEVIA
NVALEAMGHQKGEYQYLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDALLASLDSLIQAFA
AKGKEFDHVLKMGRTQLQDAVPMTLGQEFRAFATTMTEDLQRLRSLAPELLTEINLGGTA
IGTGINADPGYQALAVQRLATISGHPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKI
CNDLRLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFAIMGNDLALTVAA
EGGQLQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCIVGITANEQRCRELVEHSIGLVTA
LNPYIGYENATRIARVALESGRGVLELVREEKLLDDAMLDDILRPENMIAPRLVPLKA