Protein Info for PP_5331 in Pseudomonas putida KT2440

Annotation: Long-chain acyl-CoA thioester hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03061: 4HBT
  • amino acids 29 to 103 (75 residues), 61.6 bits, E=3.6e-21

Best Hits

Swiss-Prot: 44% identical to YCIA_ECOLI: Acyl-CoA thioester hydrolase YciA (yciA) from Escherichia coli (strain K12)

KEGG orthology group: K10806, acyl-CoA thioesterase YciA [EC: 3.1.2.-] (inferred from 92% identity to pae:PA5371)

MetaCyc: 44% identical to acyl-CoA thioesterase YciA (Escherichia coli K-12 substr. MG1655)
Acyl-CoA hydrolase. [EC: 3.1.2.20]

Predicted SEED Role

"cytosolic long-chain acyl-CoA thioester hydrolase family protein" in subsystem Serine-glyoxylate cycle

MetaCyc Pathways

• acyl-CoA hydrolysis (1/1 steps found)
• palmitoleate biosynthesis IV (fungi and animals) (1/2 steps found)
• firefly bioluminescence (2/14 steps found)
• jasmonic acid biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis
• Limonene and pinene degradation
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.-

Use Curated BLAST to search for 3.1.2.- or 3.1.2.20

Sequence Analysis Tools

See Q88C52 at UniProt or InterPro

Protein Sequence (132 amino acids)

>PP_5331 Long-chain acyl-CoA thioester hydrolase (Pseudomonas putida KT2440)
MIELEQEDPIPQGDLALQITALPRETNGFGDIFGGWLVAQMDLAGTAMASRVAGGRVATV
AIDRMAFLVPVAVGAQLSFYTQTLEIGRSSIQMMVEVWSDDPLSSEWRKVTEAVFVFVAI
DGSGRTRSVPRR