Protein Info for PP_5327 in Pseudomonas putida KT2440

Annotation: putative phosphate ABC transporter, permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 305 to 333 (29 residues), see Phobius details amino acids 353 to 374 (22 residues), see Phobius details amino acids 396 to 415 (20 residues), see Phobius details amino acids 445 to 466 (22 residues), see Phobius details amino acids 521 to 543 (23 residues), see Phobius details TIGR00974: phosphate ABC transporter, permease protein PstA" amino acids 264 to 548 (285 residues), 249.1 bits, see alignment E=2.2e-78 PF00528: BPD_transp_1" amino acids 324 to 550 (227 residues), 58.1 bits, see alignment E=5e-20

Best Hits

KEGG orthology group: K02038, phosphate transport system permease protein (inferred from 100% identity to ppu:PP_5327)

Predicted SEED Role

"Phosphate transport system permease protein PstA (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C56 at UniProt or InterPro

Protein Sequence (556 amino acids)

>PP_5327 putative phosphate ABC transporter, permease subunit (Pseudomonas putida KT2440)
MKKDSLKGWFKSGAPGVWMSGGAVAMAVIMTVGLLAVIAVRGLGHFWPADLVQATYKVPG
QADHVVIGEVVQKEEVPRARLKGAGLPVPDQGPEFMTRELIKVGNRDLNGSDFTWVVGDW
LVDEQRPADLMALERREWGNFYGYLVSVKEQGRVVAEGPAAWNELQARLKRANQLNSELQ
QLEKKDIGAINHGLERLRLQARKLELDGKLDAAAQADMDADRAELNSRYKAIEDRLTGLH
QAFSRDSLVARDGNGREVEINLSKVVHAVQPNGMSAFTKLGTYFGKVWEFLSDDPREANT
EGGIFPAIFGTVMMTLIMAVIVTPFGVLAAVYLREYARQGPVTRLIRIAVNNLAGVPAIV
YGVFGLGFFVYVLGGSIDRLFFPEALPAPTLGTPGLLWASLTLALLAVPVVIVATEEGLA
RIPRTVREGSLALGATKAETLWKIVLPMASPAMMTGMILAVARAAGEVAPLMLVGVVKLA
PSLPVDGNYPYLHLDQKIMHLGFHIYDVGFQSPNVEAARPLVYATALLLVLVIATLNLSA
VWIRNHLREKYKALDG