Protein Info for PP_5308 in Pseudomonas putida KT2440

Annotation: Protein TonB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 243 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details PF03544: TonB_C" amino acids 161 to 240 (80 residues), 55.4 bits, see alignment E=6.9e-19 TIGR01352: TonB family C-terminal domain" amino acids 162 to 240 (79 residues), 66.6 bits, see alignment E=1e-22 PF13103: TonB_2" amino acids 179 to 223 (45 residues), 28.3 bits, see alignment 1.6e-10

Best Hits

Swiss-Prot: 93% identical to TONB_PSEPU: Protein TonB (tonB) from Pseudomonas putida

KEGG orthology group: K03832, periplasmic protein TonB (inferred from 100% identity to ppu:PP_5308)

Predicted SEED Role

"Ferric siderophore transport system, periplasmic binding protein TonB" in subsystem Campylobacter Iron Metabolism or Hemin transport system or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C75 at UniProt or InterPro

Protein Sequence (243 amino acids)

>PP_5308 Protein TonB (Pseudomonas putida KT2440)
MMKTRSNMARYGGSLAIVLGVHVVAVLLTLNWSVPQAIELPPAAMMVELAPLPEPAPPPP
PKAAPQPPAPVEEPPLPKLAEAPKPKIAIPKPPKPKAKPQPPKPEKKPEPPKEAPPTEQT
VDAPPSNSPPQKSAAPAPSIASNSNALPTWQSDLLRHLAKYKRYPEDARRRGLQGMNRLR
FVVDAEGKVVSYSMAGGSGSAALDRATLEMIRRAGTVPKPPPELLNNGTIEVVAPFVYSL
DRR