Protein Info for PP_5278 in Pseudomonas putida KT2440

Annotation: 4-guanidinobutyraldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 30 to 492 (463 residues), 572.2 bits, E=3.5e-176

Best Hits

Swiss-Prot: 46% identical to ALDH2_HUMAN: Aldehyde dehydrogenase, mitochondrial (ALDH2) from Homo sapiens

KEGG orthology group: K12254, 4-guanidinobutyraldehyde dehydrogenase / NAD-dependent aldehyde dehydrogenase [EC: 1.2.1.- 1.2.1.54] (inferred from 99% identity to ppg:PputGB1_5330)

MetaCyc: 100% identical to 4-guanidinobutyraldehyde dehydrogenase (Pseudomonas putida)
Gamma-guanidinobutyraldehyde dehydrogenase. [EC: 1.2.1.54]

Predicted SEED Role

"Aldehyde dehydrogenase (EC 1.2.1.3)" in subsystem Entner-Doudoroff Pathway or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.1.3)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
• L-arginine degradation IX (arginine:pyruvate transaminase pathway) (4/4 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• superpathway of glycol metabolism and degradation (6/7 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• putrescine degradation V (2/2 steps found)
• mitochondrial NADPH production (yeast) (4/5 steps found)
• octane oxidation (4/5 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• L-arginine degradation VIII (arginine oxidase pathway) (3/4 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (3/4 steps found)
• phytol degradation (3/4 steps found)
• putrescine degradation III (3/4 steps found)
• ethanol degradation III (2/3 steps found)
• hypotaurine degradation (2/3 steps found)
• putrescine degradation IV (2/3 steps found)
• ethylene glycol degradation (1/2 steps found)
• putrescine degradation I (1/2 steps found)
• D-arabinose degradation II (2/4 steps found)
• fatty acid α-oxidation I (plants) (2/4 steps found)
• serotonin degradation (4/7 steps found)
• histamine degradation (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• noradrenaline and adrenaline degradation (8/13 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)
• dopamine degradation (2/5 steps found)
• aromatic biogenic amine degradation (bacteria) (3/8 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• alkane oxidation (1/6 steps found)
• limonene degradation IV (anaerobic) (1/7 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• superpathway of pentose and pentitol degradation (10/42 steps found)

KEGG Metabolic Maps

• 1,2-Dichloroethane degradation
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Arginine and proline metabolism
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Fatty acid metabolism
• Geraniol degradation
• Glycerolipid metabolism
• Glycolysis / Gluconeogenesis
• Histidine metabolism
• Limonene and pinene degradation
• Lysine biosynthesis
• Lysine degradation
• Naphthalene and anthracene degradation
• Propanoate metabolism
• Pyruvate metabolism
• Tryptophan metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.-, 1.2.1.3

Use Curated BLAST to search for 1.2.1.- or 1.2.1.3 or 1.2.1.54

Sequence Analysis Tools

See Q88CA3 at UniProt or InterPro

Protein Sequence (497 amino acids)

>PP_5278 4-guanidinobutyraldehyde dehydrogenase (Pseudomonas putida KT2440)
MTTLTRADWEQRAQQLKIEGRAFINGEYTDAVSGETFECLSPVDGRFLAKVASCDLADAN
RAVENARATFNSGVWSQLAPAKRKAKLIRFADLLRKNVEELALLETLDMGKPIGDSSSID
IPGAAQAIHWTAEAIDKVYDEVAPTPHDQLGLVTREPVGVVGAIVPWNFPLLMACWKLGP
ALATGNSVVLKPSEKSPLTAIRIAQLAIEAGIPAGVLNVLPGYGHTVGKALALHMDVDTL
VFTGSTKIAKQLMVYAGESNMKRIWLEAGGKSPNIVFADAPDLQAAAEAAASAIAFNQGE
VCTAGSRLLVERSIKDKFLPMVVEALKGWKPGNPLDPQTTVGALVDTQQMNTVLSYIEAG
HKDGAKLLAGGKRTLEETGGTYVEPTIFDGVTNAMRIAQEEIFGPVLSVIAFDTAEEAVA
IANDTPYGLAAGIWTSDISKAHKTARAVRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKS
LHALEKYTELKATWIKL