Protein Info for PP_5263 in Pseudomonas putida KT2440

Annotation: GGDEF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 120 to 274 (155 residues), 83 bits, see alignment E=1e-27 PF00990: GGDEF" amino acids 125 to 273 (149 residues), 110.3 bits, see alignment E=8.7e-36 PF00563: EAL" amino acids 299 to 533 (235 residues), 253.3 bits, see alignment E=2.1e-79

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5263)

Predicted SEED Role

"GGDEF domain/EAL domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CB8 at UniProt or InterPro

Protein Sequence (555 amino acids)

>PP_5263 GGDEF domain protein (Pseudomonas putida KT2440)
MSTPVEPLRLLLLADEPEWAASLHACMPALGGSAVLLTAPNWEAVDSLFSHDRQAVVLAT
PALQPAPGRCELPTILLLEHEPEAAPSAVSDWLVRDQLNADTLRRSLRHVRERGVLVATL
QRLAEQDPLTGIANRQGFQALLNARLAENEGRGVALGHLDLDNFRHVNDALGHQSGDRLI
LQVVARLKHQLEAGDQLARLGSDEFALLIDTRRDANRAEWIAERIVEALAEPYWIDGESL
LLGCSLGIAHARAQSGADPLMWHAHIAMRQAKGSQGCTFHVFNERINRNARSLADLESEL
RRALRRDELELHYQPRLNLADGRIVGLEALVRWRHAERGLLPPSEFVPLAEQSGLIVPLG
YWVISRALRDMQALCERGLEPLHMAVNLSFRQFQDSQLLATLSRLILEHGVDARWLEFEL
TETAVMRRNEVVRQTMDALGRLGVRFSLDDFGTGFSSFVHLNSLPITLLKVDRSFVAEME
MREENRKLVHAMINLAHNLNLEVVAEGVESEEQLTLLRGFGCDQVQGFLVSRPLPIGELM
AYLLQAPGRQLEGVL