Protein Info for PP_5219 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 46 to 67 (22 residues), see Phobius details amino acids 76 to 94 (19 residues), see Phobius details amino acids 134 to 160 (27 residues), see Phobius details amino acids 301 to 319 (19 residues), see Phobius details amino acids 325 to 348 (24 residues), see Phobius details amino acids 368 to 398 (31 residues), see Phobius details PF04116: FA_hydroxylase" amino acids 80 to 213 (134 residues), 115.9 bits, see alignment E=9.3e-38

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5219)

Predicted SEED Role

"Sterol desaturase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CG2 at UniProt or InterPro

Protein Sequence (431 amino acids)

>PP_5219 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MDLILLAVPFFFVLIAVELVADRVRGQRNFTLADSINSLSTGALSTSTGLLTKGVGLVTY
ALAFEHLALLQLPAQAGWVWLLAFVLYDFCYYWLHRLGHERNVLWAAHSVHHQSEEYNLT
TALRQTSSGFIFSWLFYLPLALLGVPPLVFITVASLNLLYQFWVHTRHIPKLGWLEWVLI
TPSNHRVHHAQNPAYLDRNYGGVFILWDRLFGTFKEEDPAEPVVFGVTTPLASWNPLWAN
LQFYAQLWHDARRTTSLWDKLRIWFMPTGWRPADVAARYPQAKQDLALFRKFEIALGRGQ
QAYVAAQFVVYVALGSYLMDVADRVPAAALVLGWSLMAFGLFVLGALLENRPWSARLELA
RLCLNAPALYLAGALGLAVVVPLTWLLLVAYSLLSLWGLGLARRPALRAQSTEAPAQPQQ
AAQRHQAAKHP