Protein Info for PP_5217 in Pseudomonas putida KT2440

Annotation: Polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 transmembrane" amino acids 455 to 472 (18 residues), see Phobius details PF13089: PP_kinase_N" amino acids 44 to 149 (106 residues), 124.2 bits, see alignment E=7.2e-40 TIGR03705: polyphosphate kinase 1" amino acids 44 to 717 (674 residues), 946.2 bits, see alignment E=5.4e-289 PF02503: PP_kinase" amino acids 160 to 341 (182 residues), 205.8 bits, see alignment E=1.1e-64 PF17941: PP_kinase_C_1" amino acids 368 to 531 (164 residues), 234.7 bits, see alignment E=1.1e-73 PF13091: PLDc_2" amino acids 415 to 528 (114 residues), 27.9 bits, see alignment E=4.7e-10 PF13090: PP_kinase_C" amino acids 540 to 707 (168 residues), 220.7 bits, see alignment E=2.2e-69

Best Hits

Swiss-Prot: 100% identical to PPK1_PSEPK: Polyphosphate kinase (ppk) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to ppu:PP_5217)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CG4 at UniProt or InterPro

Protein Sequence (727 amino acids)

>PP_5217 Polyphosphate kinase (Pseudomonas putida KT2440)
MLQTPPDLPPVAEPVAEQVVEAVAPVPAPSPTIAVPGLDDSSLYIHRELSQLQFNIRVLE
QALDENYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQINFAREQAGADGLQPHQALARIS
ELVHIEVERQYAILNDVLLPELEKHQIRFIRRRYWTPKLKTWVRRYFRDEIAPIITPIGL
DPTHPFPLLVNKSLNFIVELEGVDAFGRDSGLAIIPAPRLLPRVIRVPEEVGGPGANYVF
LSSMIHAHADDLFQGMKVKGCYQFRLTRNADLALDSEEVDDLARALRGELFSRRYGDAVR
LEVADTCPKHLSDYLLKQFSLSESELYQVNGPVNLTRLFSITGLDSHPELQYTPFTPAIP
KLLANADNIFSVISKQDILLMHPFESFTPVVDLLRQAAKDPHVLAVRQTLYRSGANSEIV
DALVDAARNGKEVTAVIELRARFDEESNLQMASRLQAAGAVVIYGVVGFKTHAKMMLILR
REQGEIVRYAHLGTGNYHAGNARLYTDYSLLTSDDALTEDVGKLFSQLIGMGKTLRMKKL
LHAPFTLKKGMLDMIARETQFALEGKPAHIIAKFNSLTDAKVIKALYKASQSGVKIDLVV
RGMCCLRPGIPGVSHNIQVRSIIGRFLEHTRVFYFLNGGEEQIYLSSADWMERNLDKRVE
TCFPVEGKKLLLRVKKELEGYLTDNTHAWTLQPDGRYVRSTPTGNQNPRSVQATLLERLS
NPVLNVR