Protein Info for PP_5216 in Pseudomonas putida KT2440

Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 TIGR03706: exopolyphosphatase" amino acids 12 to 306 (295 residues), 334.4 bits, see alignment E=3.2e-104 PF02541: Ppx-GppA" amino acids 26 to 309 (284 residues), 260.1 bits, see alignment E=2.8e-81 PF21447: Ppx-GppA_III" amino acids 314 to 486 (173 residues), 163.3 bits, see alignment E=5.1e-52

Best Hits

Swiss-Prot: 74% identical to PPX_PSEAI: Exopolyphosphatase (ppx) from Pseudomonas aeruginosa

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 100% identity to ppu:PP_5216)

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CG5 at UniProt or InterPro

Protein Sequence (500 amino acids)

>PP_5216 exopolyphosphatase (Pseudomonas putida KT2440)
MPHTIAKNLSLIAAIDLGSNSFHMVVAKAHHTEIRILERLGEKVQLAAGIDEERMLSEEA
MERGLDCLKRFSQLINGMPAGAVRIVGTNALREARNRNEFIQRAEAILGHPVEVISGREE
ARLIYLGVSHTLADTPGKRLVADIGGGSTEFIIGQRFEPLLRESLQMGCVSFTQRYFRDG
KITPARYAQAYTAARLELMSIENALHRLTWDEAIGSSGTIRAIGAAIKAGGLGNGEVNAE
GLAWVKRKLFKLGEVDKIDFDGVKPDRRAIFPAGLAILEAIFDALELNRMDHCDGALREG
VLFDLLGRHHHEDVRERTLNSLMERYHVDQGQAARVERKALHAFDQVADAWNLKDGNWRD
LLGWAAKVHEIGLDIAHYHYHKHGAYLIEHSDLAGFSREDQQMMALLVRGHRRNIPKDRY
AELGDEGVKLLRLCVLLRFAILFHHIRGTQQMPTVELHGGDNSLDVAFPEGWLQQNQLTQ
ADFANEAEWLARVGFVLSVR