Protein Info for PP_5212 in Pseudomonas putida KT2440

Annotation: Oxidoreductase, iron-sulfur-binding

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 PF00111: Fer2" amino acids 3 to 70 (68 residues), 40.7 bits, see alignment E=1.9e-14 PF00175: NAD_binding_1" amino acids 197 to 297 (101 residues), 59.7 bits, see alignment E=4.3e-20

Best Hits

KEGG orthology group: K00523, CDP-4-dehydro-6-deoxyglucose reductase [EC: 1.17.1.1] (inferred from 100% identity to ppf:Pput_5121)

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CG9 at UniProt or InterPro

Protein Sequence (322 amino acids)

>PP_5212 Oxidoreductase, iron-sulfur-binding (Pseudomonas putida KT2440)
MQVTLQPSGAVLALEPGERILDGARRLGYDCPNSCRNGNCHVCAALLVEGRVRQDGEVRD
HGELFTCIAEPLEDCVLLWDGVLALGELPVRKLACSVSECVDVGGDVWRVRLRAPAGKPL
RYHAGQYLMIEREGGKQAAFSLASAPHAGRELELHVLAREPSAVQLIDQLRRDGLARIEM
PFGDTHLAELPDGPLVLIAAGTGMGQMHSLVEHCRAQGFKHPVHLYWGVRRPEDFYQIEH
WDEWQRLPNLFLHQVVSDLCGWEGRCGMLHEAVCEDIADLNSVHVYASGSPNMIYATLDA
LVEAGMDAHRMRADVFAYAPRG