Protein Info for PP_5194 in Pseudomonas putida KT2440

Annotation: aminomethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to GCST_PSEPK: Aminomethyltransferase (gcvT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 100% identity to ppu:PP_5194)

MetaCyc: 57% identical to aminomethyltransferase (Escherichia coli K-12 substr. MG1655)
Aminomethyltransferase. [EC: 1.4.1.27, 2.1.2.10]; 1.4.1.27 [EC: 1.4.1.27, 2.1.2.10]

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

folate transformations III (E. coli) (9/9 steps found)
folate transformations II (plants) (10/11 steps found)
glycine biosynthesis II (3/3 steps found)
glycine cleavage (3/3 steps found)
glycine degradation (3/3 steps found)
photorespiration II (7/10 steps found)
folate transformations I (9/13 steps found)
photorespiration I (6/9 steps found)
photorespiration III (6/9 steps found)
reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 1.4.1.27 or 2.1.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CI7 at UniProt or InterPro

Protein Sequence (360 amino acids)

>PP_5194 aminomethyltransferase (Pseudomonas putida KT2440)
MGQRTLLYDLHLALGAKTVDFGGWDMPLHYGSQVEEHHQVRSDCGVFDVSHMTVIDVDGT
DATVWLQRLLANDVARLDDPGKALYSPLLNEQGGVIDDLIVYRTETGYRLVTNAATRAKV
LDWLQLQRAGFSVDFQVRPDLAILAIQGPRAREKVAALLSPARAALIRELRPFEGVADGD
WFIARTGYTGEDGLEIIFPGDQAVAFFNDLVGAGIAPSGLGARDTLRLEAGMNLYGQDID
ENHTPLTSNLGWSIAWEPAERNFIGRVGLLAEIEHGVQEKLVGLVLEERGVLRAHQVVRV
AGIGEGEITSGSFSPTLSKSIALARVPMATGDRAEVEIRGKWYPVRVVKPTFVRHGKILI