Protein Info for PP_5178 in Pseudomonas putida KT2440

Annotation: spermidine/putrescine ABC transporter - inner membrane subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 92 to 113 (22 residues), see Phobius details amino acids 123 to 144 (22 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 229 to 248 (20 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 106 to 292 (187 residues), 48.7 bits, see alignment E=3.9e-17

Best Hits

Swiss-Prot: 61% identical to POTH_ECOLI: Putrescine transport system permease protein PotH (potH) from Escherichia coli (strain K12)

KEGG orthology group: K11075, putrescine transport system permease protein (inferred from 99% identity to ppg:PputGB1_5238)

MetaCyc: 61% identical to putrescine ABC transporter membrane subunit PotH (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotH (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CK2 at UniProt or InterPro

Protein Sequence (306 amino acids)

>PP_5178 spermidine/putrescine ABC transporter - inner membrane subunit (Pseudomonas putida KT2440)
MKLRKLKRAFQRLVPEGRHLVIGVPFIWLFLFFMLPFFIVLKISFAEADVAIPPYTEIYT
YVEDKIQLVLNLANYGLLTEDELYLSAYLGSLKMAFFSTLLCLLIGFPMAYAIANAKKET
QTVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLLWTGLIDQPLQILNTNLAVYIGV
VYSYLPFMILPLFANLVKHDPSLLEAASDLGSSTFNSFWKITVPLSKNGIIAGCMLVFIP
VVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIVPILLFNRSQAK
EMEGRA