Protein Info for PP_5116 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 PF00675: Peptidase_M16" amino acids 59 to 194 (136 residues), 27.2 bits, see alignment E=3.5e-10 PF05193: Peptidase_M16_C" amino acids 203 to 378 (176 residues), 95.5 bits, see alignment E=3.7e-31

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5116)

Predicted SEED Role

"FIG146452: Zinc protease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CR4 at UniProt or InterPro

Protein Sequence (456 amino acids)

>PP_5116 conserved protein of unknown function (Pseudomonas putida KT2440)
MTDSTTLQQPAGANNLYGGLLSAQGLDLDQFETIHTQVQAWKTTAGTDVKFVEARELPIV
DVILRFKAGTTQDTLYPGLAALTLSMLDEGSQAYTAAQQAEHLERLGAVMEKQVRLEHAT
LRLRSLSPPSLLDPALAVFTDLVAHPVFHPMALTRIKRQLLQNHASRERLPILRARSEVF
RHLFNGHPYGNPLGSTAQGIEAITPEDLRAFHQRAYSASNLEMVVVGDLSPGQAQAISQQ
ISQALPQGWSATELPAAPSAPSATIAVEQAGASSAILLALPMNVPANDPEFLALALASAV
LGEGLESRLMVELRQRRGLTYGVHTHVLPLSAGGLFTVEWEVAPQHVQGTQALVETLLQA
FIDEGPTQLELQLARKQLEGQLLRGIAQNRQLATLLTEVTHQRQPADHLDTYSARIAELT
PADVRAVMQRRLALSHKVLVSVGPGVQQQPLPAPDQ