Protein Info for PP_5101 in Pseudomonas putida KT2440

Annotation: coproporphyrinogen/heterocyclic compound oxidase (aerobic)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 20 to 381 (362 residues), 398 bits, see alignment E=2e-123 PF04055: Radical_SAM" amino acids 22 to 189 (168 residues), 94.8 bits, see alignment E=6.6e-31 PF06969: HemN_C" amino acids 317 to 381 (65 residues), 54.4 bits, see alignment E=1e-18

Best Hits

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 100% identity to ppu:PP_5101)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CS9 at UniProt or InterPro

Protein Sequence (391 amino acids)

>PP_5101 coproporphyrinogen/heterocyclic compound oxidase (aerobic) (Pseudomonas putida KT2440)
MIEMLSPPGAAGFSSLPPLALYIHIPWCVRKCPYCDFNSHAAGPELPEDAYVTALLDDLD
QELAAVQGRPISSIFFGGGTPSLFSASALGRLLRGVEQRIPFAPDIEITLEANPGTFEQD
KFKAYRQTGINRLSIGVQSFQPAKLEALGRIHNGDEAVRAAGMARAAGFDNFNMDLMHGL
PDQSLDDALGDLRQAIDLGPTHLSWYQLTVEPNTVFWNQPPELPEDDILWDIQEAGQALM
AEHGYRQYEVSAYAQAGRAARHNLNYWRFGDFIGIGAGAHGKLTFADGRILRTWKTRLPK
DYLNLAKPFRAGEKLLPVDELPFEFLMNALRLTDGVEAELFTQRTGLPLAQLQEARRAAE
QKGLLQVEPDRLVATPRGQLFLNDLLQYFLT