Protein Info for PP_5089 in Pseudomonas putida KT2440

Annotation: arginyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 PF03485: Arg_tRNA_synt_N" amino acids 4 to 90 (87 residues), 89.4 bits, see alignment E=2.9e-29 TIGR00456: arginine--tRNA ligase" amino acids 6 to 578 (573 residues), 564.7 bits, see alignment E=1e-173 PF00750: tRNA-synt_1d" amino acids 101 to 448 (348 residues), 437.1 bits, see alignment E=6.7e-135 PF05746: DALR_1" amino acids 462 to 578 (117 residues), 115.9 bits, see alignment E=1.7e-37

Best Hits

Swiss-Prot: 100% identical to SYR_PSEPK: Arginine--tRNA ligase (argS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 100% identity to ppu:PP_5089)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CU1 at UniProt or InterPro

Protein Sequence (578 amino acids)

>PP_5089 arginyl-tRNA synthetase (Pseudomonas putida KT2440)
MKDTIRQLIQQALTQLVTDGVLPEGLSPAIQVENARDKTHGDFASNIAMMLAKPAGMKPR
DLAEKLINALPASADISKVEIAGPGFLNFFQNTDALANRLDAALADAHLGARKAGPAQKV
VIDMSAPNLAKEMHVGHLRSTIIGDSVARVLEFLGDNVIRQNHVGDWGTQFGMLMAYLQE
NPITSDELSDLENFYRAAKKRFDESEEFATRARGLVVKLQAGDPECLALWTRFKDISLSH
CQKTYELLNVKLTMADVMGESAYNDDLANVVADLKAKGLLVEDQGAQCVFLEEFKNSDGD
PLPVIVQKADGGYLYATTDLAAVRYRSNVLKADRALYFVDQRQALHFNQVFEVARRAGFV
GHPMQMEHMGFGTMNGADGRPFKTRDGGTVKLIDLLTEAKERAYALVKEKNPSLADDELR
HIGEVVGIGAVKYADLSKHRTSDYSFNFELMLNFEGNTAPYLLYAYTRVAGVFRKLGKGF
DEVDGKIVLQAAHEQDLAARLAQFGEILNNVAEKGTPHVLCSYLYDLAGLFSSFYENCPI
LAAETPSQQQSRLRLAALTGRTLKQGLELLGLETLERM