Protein Info for PP_5025 in Pseudomonas putida KT2440
Annotation: Glucans biosynthesis glucosyltransferase H
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to OPGH_PSEPK: Glucans biosynthesis glucosyltransferase H (opgH) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K03669, membrane glycosyltransferase [EC: 2.4.1.-] (inferred from 100% identity to ppu:PP_5025)Predicted SEED Role
"Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-)" (EC 2.4.1.-)
KEGG Metabolic Maps
- Anthocyanin biosynthesis
- Carotenoid biosynthesis - General
- Flavone and flavonol biosynthesis
- Flavonoid biosynthesis
- Fructose and mannose metabolism
- Glycerolipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- High-mannose type N-glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Peptidoglycan biosynthesis
- Sphingolipid metabolism
- Zeatin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.-
Use Curated BLAST to search for 2.4.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88D04 at UniProt or InterPro
Protein Sequence (857 amino acids)
>PP_5025 Glucans biosynthesis glucosyltransferase H (Pseudomonas putida KT2440) MSNSSARPESLGEYLAHLPLSDEQRAELASCTSFSELHQRLAANPAASSAEAVQASVGPR LTVGSAAELEEAEMLGVDGSGRLCLKIAPPIKRTKVVPEPWRTNVLIRMWRRMTGRPNAP QPPKRELPPARWRTVGSIRRYILLALMIGQTIVAGWYMKGILPYQGWSFVDFDEIVNQPL WDTVVQVWPYALQTSILILFGILFCWVSAGFWTALMGFLELLTGRDKYKISGSSAGNEPI APEARTALVMPICNEDVPRVFAGLRATFESVAASGNLDRFDFFVLSDTNDTDIAVAEQQA WLDVCRETKGFGRIFYRRRRRRVKRKSGNLDDFCRRWGGEYKYMVVLDADSVMSGECLSS LVRLMEANPDAGIIQTGPKASGMDTLYARMQQFATRVYGPLFTAGLHFWQLGESHYWGHN AIIRMKPFIEHCALAPLPGKGAFAGAILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPP NLLDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFLFLVLSTALL ATNTLMEPQYFIEPYQLYPLWPQWHPEKAVALFSTTIVLLFLPKLLSVILIWAKGAVEYG GRVKVTLSMLMEMLFSMLLAPVRMIFHTRFVLAAFLGWAATWNSPQRDDDSTPWGEAVRR HGPQTLLGIAWAALVAWLNPSFLWWLAPIVGSLVLSIPVSVISSRTRLGLAAKDEKLFLI PEEYATPQELLATDQYTHENRWHALHDGFVRAVVDPRQNALACAMATARHGQAAPIEALR AERVAKAIEVGPKGLDLNTRLALLSDPVALSRLHEQVWAEHNAAWIDVWRASINNDPHSP LLPLHPENAGQPSLVGA