Protein Info for PP_5007 in Pseudomonas putida KT2440

Annotation: Polyhydroxyalkanoate granule-associated protein GA2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 PF05597: Phasin" amino acids 9 to 132 (124 residues), 149 bits, see alignment E=3.6e-48 TIGR01837: poly(hydroxyalkanoate) granule-associated protein" amino acids 18 to 133 (116 residues), 144.6 bits, see alignment E=7e-47

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5007)

Predicted SEED Role

"Polyhydroxyalkanoate granule-associated protein PhaF" in subsystem Polyhydroxybutyrate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D21 at UniProt or InterPro

Protein Sequence (261 amino acids)

>PP_5007 Polyhydroxyalkanoate granule-associated protein GA2 (Pseudomonas putida KT2440)
MAGKKNTEKEGSSWVGGIEKYSRKIWLAGLGIYSKIDQDGPKLFDSLVKDGEKAEKQAKK
TAEDVAETAKSSTTSRVSGVKDRALGKWSELEEAFDKRLNSAISRLGVPSRNEIKALHQQ
VDSLTKQIEKLTGASVTPISSRAAATKPAASKAAAKPLAKAAAKPAAKTAAAKPAGKTAA
AKPAAKTAAEKPAAKPAAKPAAAKPAAAKKPAVKKAPAKPAAAKPAAPAASAAPAATTAP
ATAATPASSTPSAPTGTGTLI