Protein Info for PP_4954 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 transmembrane" amino acids 21 to 38 (18 residues), see Phobius details amino acids 44 to 60 (17 residues), see Phobius details amino acids 68 to 85 (18 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 116 to 134 (19 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details amino acids 422 to 438 (17 residues), see Phobius details amino acids 445 to 463 (19 residues), see Phobius details amino acids 469 to 486 (18 residues), see Phobius details amino acids 492 to 510 (19 residues), see Phobius details amino acids 516 to 538 (23 residues), see Phobius details TIGR01667: integral membrane protein, YccS/YhfK family" amino acids 12 to 719 (708 residues), 918.9 bits, see alignment E=1.9e-280 TIGR01666: TIGR01666 family membrane protein" amino acids 14 to 717 (704 residues), 753.7 bits, see alignment E=1.8e-230 PF12805: FUSC-like" amino acids 71 to 355 (285 residues), 372.8 bits, see alignment E=1.9e-115 PF04632: FUSC" amino acids 399 to 705 (307 residues), 48.3 bits, see alignment E=1.3e-16 PF13515: FUSC_2" amino acids 413 to 532 (120 residues), 88 bits, see alignment E=1.2e-28

Best Hits

KEGG orthology group: None (inferred from 99% identity to ppf:Pput_4827)

Predicted SEED Role

"FIG00956403: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D73 at UniProt or InterPro

Protein Sequence (727 amino acids)

>PP_4954 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MSSSSFRQSLRRLWGQDKFSYSIRVTIALTGSLALCWYQNEMALLIPLFLGIIASALAET
DDSWQGRLSALAVTLVCFAIAALAVELLFPYPWIFVVALALAAFGLTMLGALGERYGAIA
SATLITAVYTMIGVDQRGGQVTDFWHEPLLLVAGAAWYGLLSVLWQALFSNQPVQQSLAR
LFFELGSYLKLKASLFEPVRTLDVEARRLELAQQNGKVVAALNAAKEIILHRVGNSQPNS
KVSRYLKLYFLAQDIHERVSASHYPYNALTEAFFHSDVMFRCQRLLRKQGSSCQELARSI
RLRQPFVLASGYPEALEDLNASLEHLRIQSNPAWRGLLRSLRALAANLATLDRLLSAASN
PDSLADASDSSLLDRSPRSLKDVWTRLRTQLTPTSLLFRHALRLPLALSIGYGMVHLIHP
TQGYWIILTTLFVCQPNYGATRRKLVQRIFGTAVGLTVGWALFDLFPNPVIQSLFAVVAG
VVFFVNRTTRYTLATAAITLMVLFCFNQIGDGYGLFLPRLFDTLVGSLIAILAVFLFLPD
WQGRRLNKALANTLACASVYLRQIMQQYAHGKRDDLAYRLARRNAHNADAALSTTLANML
MEPGHFRKEADVGFRFLVLSHTLLSYLSGLGAHRDTALPAEVQEQLIEGAGQSLASSLDE
IANGLAARLPVAIHSDSEEALANALEQMPEDLDEHQRLVQTQLALICRQLGPLRTLAAHL
IKENAPA