Protein Info for PP_4951 in Pseudomonas putida KT2440

Annotation: Drug resistance transporter, EmrB/QacA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 53 to 73 (21 residues), see Phobius details amino acids 81 to 104 (24 residues), see Phobius details amino acids 106 to 128 (23 residues), see Phobius details amino acids 139 to 161 (23 residues), see Phobius details amino acids 167 to 188 (22 residues), see Phobius details amino acids 200 to 219 (20 residues), see Phobius details amino acids 229 to 248 (20 residues), see Phobius details amino acids 268 to 292 (25 residues), see Phobius details amino acids 299 to 318 (20 residues), see Phobius details amino acids 329 to 352 (24 residues), see Phobius details amino acids 358 to 378 (21 residues), see Phobius details amino acids 398 to 423 (26 residues), see Phobius details amino acids 436 to 459 (24 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 18 to 418 (401 residues), 252.2 bits, see alignment E=5.1e-79 PF07690: MFS_1" amino acids 19 to 239 (221 residues), 118 bits, see alignment E=2.4e-38 amino acids 273 to 467 (195 residues), 40 bits, see alignment E=1.2e-14

Best Hits

Swiss-Prot: 50% identical to HSRA_ECOLI: Probable transport protein HsrA (hsrA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4951)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D76 at UniProt or InterPro

Protein Sequence (475 amino acids)

>PP_4951 Drug resistance transporter, EmrB/QacA family (Pseudomonas putida KT2440)
MPERTPLDPVTARWIPWVVAIAFFMQSLDGTILNTALPAMARSLAEDPLRMQGVIIAYML
TVALLIPASGWIADRFGTKRIFFSAILLFSFGSLLCAAANSLGFLIFARVVQGLGGALML
PVGRLVVLRAYPRSELVRIMSFITIPGLLGPLLGPTVGGWLVEILSWHWIFLLNLPVGLL
GCIAVWKFIPDLRGAERTTFDGPGFLLFGAAMVLITIAMEGLGELHLPHLRVMLLLFAGM
ACLAAYWLRAGRDPEPLFSPSLFRVRTFAIGILGNLFARLGSGALPFLVPLLLQVALGYS
PAQAGMSMIPLAAAAMLAKSIARPLIERFGYRIILTGNTLLLGILLASLGLVDEQTPYWL
LLVQLGLLGAVNSMQFTAMNTVTLIDLDDASASSGNSLLSVVAQLSLSLGVACAGALLGG
FTAAGSAEGVETTLGAFQLTFVTIGVMAMLAAAIFLQLAPTDGRRARRPEQHMES