Protein Info for PP_4946 in Pseudomonas putida KT2440

Annotation: sodium/L-proline transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 transmembrane" amino acids 54 to 75 (22 residues), see Phobius details amino acids 115 to 139 (25 residues), see Phobius details amino acids 172 to 199 (28 residues), see Phobius details amino acids 211 to 232 (22 residues), see Phobius details amino acids 240 to 260 (21 residues), see Phobius details amino acids 280 to 298 (19 residues), see Phobius details amino acids 323 to 345 (23 residues), see Phobius details amino acids 373 to 397 (25 residues), see Phobius details amino acids 419 to 439 (21 residues), see Phobius details amino acids 446 to 470 (25 residues), see Phobius details amino acids 477 to 493 (17 residues), see Phobius details amino acids 500 to 520 (21 residues), see Phobius details TIGR02121: sodium/proline symporter" amino acids 55 to 536 (482 residues), 741 bits, see alignment E=6.1e-227 TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 83 to 485 (403 residues), 394.6 bits, see alignment E=5.8e-122 PF00474: SSF" amino acids 83 to 485 (403 residues), 450.6 bits, see alignment E=2.5e-139

Best Hits

Swiss-Prot: 75% identical to PUTP_SALTY: Sodium/proline symporter (putP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K11928, sodium/proline symporter (inferred from 100% identity to ppf:Pput_4818)

MetaCyc: 76% identical to proline:Na+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-118; TRANS-RXN0-505

Predicted SEED Role

"Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter" (TC 2.A.21.2.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D81 at UniProt or InterPro

Protein Sequence (542 amino acids)

>PP_4946 sodium/L-proline transporter (Pseudomonas putida KT2440)
MGFVGKCTGRCNLQKKNGCTFFALLHSIRRLGATTNGVVAHDKNKRQGTRMGNPLTITFV
IYIAAMVLIGFAAYRATNNLSDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYFAG
LSEAWIAIGLTVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDNSGLLRIISAIV
ILVFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGFLAVSWTDTVQAS
LMIFALILTPVIVLISTGGFDQTFAAIEAVNPANFDMLKGATFIGIISLMGWGLGYFGQP
HILARFMAADSVKSIAKARRISMTWMILCLAGTCAVGFFGIAYFSAHPELAGPVSENHER
VFIELAKILFNPWVAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAFLRKNASQVELV
WVGRLMVLAVALIAIAMAANPENRVLGLVAYAWAGFGAAFGPVVLISVLWKGMTRNGALA
GIVVGALTVILWKNFDTLGLYEIIPGFLFASIAIVLVSKLGSPSQTMVKRFEAADAAYHA
DK