Protein Info for PP_4927 in Pseudomonas putida KT2440

Annotation: Cyclolysin secretion/processing ATP-binding protein CyaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 731 transmembrane" amino acids 175 to 197 (23 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 290 to 309 (20 residues), see Phobius details amino acids 315 to 338 (24 residues), see Phobius details PF03412: Peptidase_C39" amino acids 28 to 148 (121 residues), 70.2 bits, see alignment E=2.4e-23 TIGR01846: type I secretion system ATPase" amino acids 32 to 727 (696 residues), 1002 bits, see alignment E=7.3e-306 PF00664: ABC_membrane" amino acids 177 to 443 (267 residues), 193.1 bits, see alignment E=1.3e-60 PF00005: ABC_tran" amino acids 507 to 655 (149 residues), 117.3 bits, see alignment E=1.2e-37

Best Hits

Swiss-Prot: 62% identical to CYAB_BORPE: Cyclolysin secretion/processing ATP-binding protein CyaB (cyaB) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K11004, ATP-binding cassette, subfamily B, bacterial HlyB/CyaB (inferred from 100% identity to ppu:PP_4927)

Predicted SEED Role

"cyclolysin secretion ATP-binding protein" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DA0 at UniProt or InterPro

Protein Sequence (731 amino acids)

>PP_4927 Cyclolysin secretion/processing ATP-binding protein CyaB (Pseudomonas putida KT2440)
MLRASIASCPVFIRGGVMADLSDGNANTIESGLVCLITLARYHGVAVSAEQLQHDHAAAG
ERFSTGPLLRAFQQLGLKAKHRSVDPARLQQTPLPAIAVDTRGEYFIIARVEGEQVLVQD
PHSRAPQALTLAELLVRWSGELILVRSDAQLPLGLSRFDFTWFIPALVKYRRLFGEVLVV
SLVIQILALLTPLFFQVVMDKVLVHRGLTTLDVIALGLLVVVLFETLLSGLRTYVAAHTA
SRIDVELGAKLFRHLIDLPTAYFHARRVGDSVARVRELENIRSFLTNNSITLVLDVLFSV
VFIAVMFLYSGWLTLVVVGSLPFYFLVSLVVTPLLRALIDQSFQRGAENQAFLVEAVNGI
DTLKSMAVEPQVTRRWNDQLAAYVSASFKTQNLSSLANESVGLIGKLVTVATLWLGARLV
IEGALTVGELIAFNMLAGRVSQPIIRLAQLWTSFQQTGVSIQRLGDILNTRTEVNQGKGT
VLPTLVGQIELDRVCFRYRPDGGEVVRDVSLKIAAGEVIGVVGRSGSGKSTLTRLIQRLY
SPERGRVLPDGADLAVADVASLRRQIGVVLQDNVLFRRTVRENIALGNPAAPLEEVIAAA
TLAGAHEFILELPDGYETLIGEHGASLSGGQRQRIAIARALFGNPRILIFDEATSALDYE
SERIIQQNMARICAQRTVIIIAHRLSAVRHADRIVVMERGQIIEQGTHDELLGYPQGTYA
YLHQLQLGAAV