Protein Info for PP_4897 in Pseudomonas putida KT2440

Annotation: N-acetylmuramoyl-L-alanine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF11741: AMIN" amino acids 30 to 128 (99 residues), 77 bits, see alignment E=1.6e-25 PF01520: Amidase_3" amino acids 180 to 398 (219 residues), 158.4 bits, see alignment E=2.8e-50 PF01476: LysM" amino acids 427 to 469 (43 residues), 45.1 bits, see alignment 1.2e-15

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 100% identity to ppf:Pput_4773)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DD0 at UniProt or InterPro

Protein Sequence (476 amino acids)

>PP_4897 N-acetylmuramoyl-L-alanine amidase (Pseudomonas putida KT2440)
MRIRALVAIVGLLLTTVTVEALAVTQVKSMRLWRAPDNTRLVFDLSGPVQHSVFTLSAPD
RLVIDINGATLAAPLNVATSNTPISSVRSAQRTPTDLRVVVDLKKSVTPKSFTLAPNAQY
GNRLVVDLYDQEADAIAASAPPPAPAPVQTPATTPAVPVTPAQPAIKLPPVPSGKRDIVV
AIDAGHGGEDPGASGSRGQHEKDIVLQIAKELQRQINSEKGFRAELTRTGDYFIPLRKRT
EIARKKGADLFISIHADAAPSRAAFGASVFALSDRGATSETARWLADTENRSDLIGGAGN
VSLDDKDRMLAGVLLDLSMTATLSSSLNVGQKVLGNMGRVTSLHKQRVEQAGFMVLKSPD
IPSILVETGFISNNNEAAKLASSSHQQALARSIRTGVHQYFQQNPPPGTYIAWLRDTGKI
AAGPREHTVRPGETLAMLAVRYQVSVASLRSINSLKTDELKVGQRLDVPATTLAAQ