Protein Info for PP_4821 in Pseudomonas putida KT2440

Annotation: putative Fis-like DNA-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 106 PF02954: HTH_8" amino acids 63 to 103 (41 residues), 50.6 bits, see alignment E=6.5e-18

Best Hits

Swiss-Prot: 88% identical to FISL_PSEAE: Putative Fis-like DNA-binding protein (PA4853) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03557, Fis family transcriptional regulator, factor for inversion stimulation protein (inferred from 97% identity to pen:PSEEN4862)

Predicted SEED Role

"DNA-binding protein Fis" in subsystem DNA structural proteins, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DK4 at UniProt or InterPro

Protein Sequence (106 amino acids)

>PP_4821 putative Fis-like DNA-binding protein (Pseudomonas putida KT2440)
MTMMTENFVSGTTPVSDNANLKQHLNTPSEEGQTLRDSVEKALHNYFAHLEGATVTDVYN
LVLSEVEAPLLESVMNYVKGNQTKASEMLGLNRGTLRKKLKQYDLL